来自Faunimonas pinastri A52C2的新型D-醇脱酶的表征

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Shuangshuang Yu^1,2,3, Youran Li^1,2,3, Guiyang Shi^4,5,6

¹Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, China.

Applied microbiology and biotechnology

|January 27, 2025

概括

从Faunimonas pinastri发现了一种新的D-醇脱酶 (SLDH),并进行了鉴定和特征. 这种酶Fpsldh使用NAD+/NADP+共酶有效地产生L-,为工业应用提供了一种新的生物催化剂.

科学领域:

生物催化剂是一种生物催化剂.
酶学是一种酶学.
微生物生物技术微生物生物技术

背景情况:

D-sorbitol脱酶 (SLDH) 酶对于将D-sorbitol转化为L-sorbose至关重要.
除了Gluconobacter oxydans之外的其他来源对SLDH的知识,包括基质特异性和辅酶需求,是有限的.

研究的目的:

为了识别和描述来自Faunimonas pinastri A52C2.2.的新型SLDH.
评估基因改造的Bacillus licheniformis表达这种新型SLDH的潜力,以生产L-酸盐.

主要方法:

BLASTp分析用于选一种新型SLDH (Fpsldh).
纯化Fpsldh的特点是其酶特性,包括辅酶依赖性,基质特异性,最佳pH和温度以及动态参数.
一个Bacillus licheniformis宿主被设计成表达Fpsldh用于全细胞生物催化.

主要成果:

一种新的NAD+/NADP+依赖的SLDH,Fpsldh,从Faunimonas pinastri A52C2.2中被发现并进行了鉴定.
在pH值8.0-10.0和温度为27-37°C时,Fpsldh表现出最佳活性,Km为7.51mM,不需要金属离子.
在没有外源辅助因子的情况下,工程化表达Fpsldh的Bacillus licheniformis在33.6小时内实现了13.19g/L的L-糖产量.

结论:

Fpsldh代表了一种具有独特催化性能的新型SLDH,扩大了这种酶家族已知的多样性.
工程化表达Fpsldh的Bacillus licheniformis提供了一种高效且独立于辅因子的全细胞生物催化剂,用于生产L-糖.
这项研究为商业生产L-酸盐提供了一种有价值的新方法.

关键词:

细菌类类类这是一种D-酸醇.D-sorbitol脱基酶的使用方法这种L-酸盐是L-酸盐.

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