工程酵母可以从铁酸和红素中生产fraxetin
Bo-Tao He1,2, Bing-Zhi Li3,4
1Frontiers Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, China.
Applied microbiology and biotechnology
|January 27, 2025
概括
工程酵母现在可以从素衍生物中产生fraxetin,一种有价值的黄类化合物. 这一突破提供了一种可持续的方法,用于素的价值化和生物活性化合物的生产.
科学领域:
- 生物技术是生物技术.
- 合成生物学 合成生物学
- 化学工程是化学工程的重要组成部分.
背景情况:
- 素是丰富的芳香化合物的来源,在生物提炼中未得到充分利用.
- 弗拉克西是一种类黄,具有显著的抗微生物,抗癌,抗氧化,抗炎和神经保护性质.
- 开发绿色和附加值的素应用对于可持续的生物提炼至关重要.
研究的目的:
- 设计Saccharomyces cerevisiae作为一个细胞工厂,用于将红素衍生物转化为fraxetin.
- 为了建立一个生物转化途径,从素单体中生产fraxetin.
- 通过微生物生产生物活性化合物来证明素增值的可行性.
主要方法:
- 工程Saccharomyces cerevisiae与 scopoletin 8-hydroxylase (S8H) 和氨酸合成酶 (COSY) 的基因.
- 优化发酵策略,以从酸中增强fraxetin的生产.
- 在工程酵母中利用氨酸解剂作为fraxetin生物合成的基质.
主要成果:
- 经过工程设计的S. cerevisiae成功地从ferulic acid中产生fraxetin,这是一个关键的素单体.
- 经过优化发酵,从ferulic acid中获得了19.1 mg/L的fraxetin标位.
- 工程酵母细胞工厂生产了7.7毫克/升的fraxetin,直接来自氨酸解剂.
结论:
- 通过使用工程化S. cerevisiae,成功地将红素单体和化物转化为fraxetin.
- 这项研究提出了一种可行的战略,用于将红素转化为高价值的生物活性化合物.
- 工程酵母作为一个可持续的细胞工厂,从可再生的素资源生产fraxetin.
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