机器学习算法的比较分析用于将aptamer-antigen结合动态配置文件转化为诊断决策
Sadman Sakib1, Kulmanak Bajaj2, Payel Sen1
1Department of Engineering Physics, McMaster University, 1280 Main Street West, L8S 4L8 Hamilton, Ontario, Canada.
ACS sensors
|January 27, 2025
概括
机器学习 (ML) 显著改善了用于诊断的生物传感器数据分类. 这一框架提高了使用人工神经网络 (ANN) 和随机森林 (RF) 的COVID-19检测准确度,超出了传统方法.
科学领域:
- 生物医学工程 生物医学工程
- 机器学习应用 机器学习应用
- 生物传感器技术技术
背景情况:
- 传统的生物传感器数据分类使用固定值 (ROC曲线),限制了复杂信号的准确性.
- 诊断中的实时电化学测量需要先进的分析方法.
- 现有的方法在高变量生物传感器数据上扎,影响诊断可靠性.
研究的目的:
- 开发和验证一种机器学习 (ML) 框架,用于增强生物传感器诊断数据的二进制分类.
- 为了提高复杂和可变的电化学信号的诊断准确度.
- 将ML应用于实时多重体体测定 (RT-MAp) 进行病毒蛋白检测.
主要方法:
- 开发了一个框架,用于将ML应用于实时电化学生物传感器数据.
- 通过非线性回归,从172个COVID-19唾液样本的短暂阻抗信号中提取了9个关键特征.
- 监督的ML算法 (SVM,ANN,RF) 在提取的特征上进行训练和测试.
主要成果:
- 机器学习模型显著优于基于ROC的传统分类 (83.6%的准确率).
- 支持矢量机 (SVM) 实现了86.0%的准确性.
- 人工神经网络 (ANN) 和随机森林 (RF) 模型实现了100%的准确性.
- 通过高变量生物传感器数据,ANN表现出卓越的性能.
结论:
- 开发的ML框架为改善诊断准确性提供了强大而可扩展的解决方案.
- 从生物传感器数据中,ANN和RF模型提供了非常准确的临床样品二元分类.
- 这种方法提高了使用电化学生物传感器的临床诊断的可靠性.
相关概念视频
01:22Protein-Drug Binding: Determination Methods
109
Determining protein-drug binding can be achieved through indirect and direct methods, each providing valuable insights into the interaction between proteins and drugs.
Indirect methods involve isolating the bound drug from its free form in biological samples such as blood, serum, or plasma. These techniques aim to measure the percentage of drugs bound to proteins. Equilibrium dialysis is a commonly used method where the free drug concentration at equilibrium is measured by separating the bound...
Indirect methods involve isolating the bound drug from its free form in biological samples such as blood, serum, or plasma. These techniques aim to measure the percentage of drugs bound to proteins. Equilibrium dialysis is a commonly used method where the free drug concentration at equilibrium is measured by separating the bound...
109
01:33Enzyme-Linked Immunosorbent Assay
12.4K
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...
12.4K