在用Lactiplantibacillus plantarum发酵百合花果粉时,水溶性多糖的积累涉及内葡萄糖酶的表达
Guitao Huang1,2, Dongxiao Su3, Yuan-Kun Lee4
1Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, P. R. China.
Journal of agricultural and food chemistry
|January 27, 2025
概括
在用 Lactiplantibacillus plantarum 发酵肉时,通过降解不溶于水的多糖化合物 (WISP),显著增加水溶性多糖化合物 (WSP). 特定的酶,如内葡萄糖酶和银河酶驱动这种转化.
科学领域:
- 食品微生物学 食品微生物学
- 生物化学 生物化学
- 酶学 是一种酶学.
背景情况:
- 果肉的发酵可以改变多糖化合物的组成.
- 了解多糖转化机制对于食品加工至关重要.
- 乳酸植物菌 (Lactiplantibacillus plantarum) 是一种常见的细菌,用于食品发酵.
研究的目的:
- 为了研究在莱奇果肉发酵过程中多糖转化的机制,由 Lactiplantibacillus plantarum ATCC 14917.14917.
- 确定负责增加水溶性多糖体 (WSP) 和降解水不溶性多糖体 (WISP) 的特定酶.
主要方法:
- 使用Lactiplantibacillus plantarumATCC 14917发酵果果粉的发酵方法
- 对多糖分 (WSP和WISP) 组成的分析.
- 碳水化合物活性酶编码序列的基因组分析.
- 转录试验, prokaryotic 表达和酶水解.
主要成果:
- 发酵导致WSP的2.32-2.67倍增加和WISP的降解.
- WISP是由葡萄糖,阿拉比诺斯,银河酸和银河糖组成的.
- 三种内葡萄糖酶被确定为降解WISP的关键酶,有助于WSP积累.
- Galactosidases 有助于减少 WSP 中的银河糖和银河酸.
结论:
- 基于酶的机制解释了L. plantarum在桃肉质发酵期间的WSP积累.
- 这些发现为食品应用中有针对性的多糖化物转化提供了基础.
- 这项研究阐明了在发酵过程中参与水果多糖体修饰的酶途径.
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