PgpP是一种广泛保守的酸酶,对于酸糖醇脂质合成而言,它是必需的
Angelika Gründling1,2, Anna P Brogan2, Michael J James2
1Section of Molecular Microbiology and Centre for Bacterial Resistance Biology, Department of Infectious Disease, Imperial College London, London SW7 2AZ, United Kingdom.
在细菌中,酸糖醇 (PG) 对于生长至关重要. 一种新的酶,PgpP,对于格拉姆阳性细菌的PG产生至关重要,与大肠杆菌不同,是潜在的抗生素标.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 酸糖醇 (PG) 是细菌细胞质膜的关键组成部分,对细胞生长至关重要.
- 虽然在大肠杆菌中PG生物合成是众所周知的,但在格拉姆阳性细菌中,终端步骤仍然不清楚.
- 大肠杆菌利用三种冗余酸酶将酸丁糖 (PGP) 转化为PG.
研究的目的:
- 为了阐明酸糖醇 (PG) 合成在格拉姆阳性细菌的机制.
- 为了确定负责Bacillus subtilisPG生产的最后一步的酶.
- 评估PgpP作为抗生素点的潜力.
主要方法:
- 细菌细菌和大肠杆菌的遗传分析.
- 基因表达和补充研究.
- 细菌生长,膜脂质含量和PGP积累的分析.
主要成果:
- 大肠杆菌PG生成酶的同类物对于Bacillus subtilis的PG合成并不必不可少.
- 一种以前未被描述的蛋白质,YqeG (重新命名为PgpP),被确定为在B. subtilis.中将PGP转化为PG的必需物.
- 来自B. subtilis和Staphylococcus aureus的PGpP可以在缺乏本地酶的大肠杆菌中挽救PG合成.
- 在B. subtilis中PgpP的耗尽导致生长停止,膜脂减少和PGP积累.
- PgpP被保存在Firmicutes,Cyanobacteria中,并且在酵母线粒体和植物质体中发现.
结论:
- PgpP是Bacillus subtilis和其他グラム阳性细菌中脂糖醇 (PG) 合成的必需酶.
- PgpP代表了一种具有古老起源的保存酶,可能参与不同生物体的脂质代谢.
- 在许多グラム阳性病原体中,PgpP是必不可少的,这使得它成为新型抗生素开发的有希望的目标.
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