聚烯微塑料减弱了 perfluorobutanoic 酸对Chlorella sorokiniana的影响:异构聚合,生物可用性,生理学和转录学
Zhilin Zhao1, Xiaoying Zheng1, Yue Li1
1Ministry of Education Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Hohai University, Nanjing 210098, PR China; College of Environment, Hohai University, Nanjing 210098, PR China.
Journal of hazardous materials
|January 27, 2025
概括
聚烯微塑料 (PS) 和 perfluorobutanoic 酸 (PFBA) 损害了藻类的生长. 由于藻类产生更多的EPS,它们的综合效应较少有毒,从而降低了污染物的生物可用性.
科学领域:
- 环境科学 环境科学
- 生态毒理学 生态毒理学
- 海洋生物学 海洋生物学
背景情况:
- 微塑料 (MP) 和 perfluorobutanoic acid (PFBA) 是已知有毒性的广泛存在的环境污染物.
- MPs和PFBA对水生生物的综合生态毒理影响仍然在很大程度上未被探索.
- 了解这些相互作用对于准确的环境风险评估至关重要.
研究的目的:
- 研究聚烯微塑料 (PS) 和PFBA对微藻Chlorella sorokiniana的单独和联合影响.
- 阐明毒性和适应的潜在细胞和分子机制.
- 评估PS和PFBA之间的对抗或协同相互作用的潜力.
主要方法:
- 暴露Chlorella sorokiniana不同度的PS微塑料和PFBA,单独或组合.
- 评估细胞反应,包括活性氧物种 (ROS) 生成,细胞膜损伤和光合作用效率.
- 转录组分析以确定关键的代谢途径和基因表达变化.
主要成果:
- 无论是PS还是PFBA,都通过诱导ROS,破坏细胞膜和损害光合作用来单独抑制Chlorella sorokiniana的生长.
- 小颗粒大小的PS表现出最高的个体毒性.
- 联合暴露显示出对抗作用,与单个暴露相比,毒性降低.
- 反对性与藻类增加的细胞外聚合物物质 (EPS) 分泌有关,导致PFBA吸附和PS异质聚合,从而降低生物可用性.
- 转录组分析显示高调节碳水化合物,氨基酸和能量代谢,促进EPS分泌,ABC运输和抗氧化剂反应.
结论:
- 菌索罗基尼亚纳表现出适应性反应,包括增强的EPS分泌和抗氧化机制,以减轻同时暴露的PS和PFBA的毒性.
- 聚氨酸微塑料和PFBA之间的相互作用导致生物利用率降低和对抗生态毒性.
- 这些发现为了解与混合微塑料和和多基物质 (PFAS) 污染相关的环境风险提供了关键数据.
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