玉米转录因子ZmEREB167负面调节粉积累和粒大小
Xiangyu Qing1, Jianrui Li2, Zhen Lin1
1State Key Laboratory of Maize Bio-breeding, Key Laboratory of Genome Editing Research and Application, Ministry of Agriculture and Rural Affairs, Department of Plant Genetics and Breeding, National Maize Improvement Center, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
Journal of genetics and genomics = Yi chuan xue bao
|January 27, 2025
概括
研究人员确定了ZmEREB167,这是一种对玉米核发育至关重要的基因. 它的淘汰会通过抑制参与内体生长的关键基因来增强内核大小,重量和营养含量.
科学领域:
- 植物分子生物学 植物分子生物学
- 玉米的遗传学 玉米的遗传学
- 农业科学 农业科学
背景情况:
- 转录因子调节玉米核发育中的基因表达.
- 玉米内对于粉存储至关重要,占成熟核干重的90%.
研究的目的:
- 为了识别和描述内精特异性的EREB基因ZmEREB167.7.的功能.
- 研究ZmEREB167在调节玉米内的发育和营养积累方面的作用.
主要方法:
- 基因鉴定和淘汰 (Zmereb167-C1突变体).
- 在现场混合化用于表达式分析.
- 对于转录活动的双露西法酶测定.
- 用于转录组和基因分析的RNA-seq和ChIP-seq.
主要成果:
- ZmEREB167是一种内精特异性,核局部化转录因子.
- ZmEREB167淘汰赛增加了内核大小,重量,粉和蛋白质含量.
- ZmEREB167作为一个转录抑制剂,降低了像OPAQUE2,ZmNRT1.1,ZmIAA12,ZmIAA19和ZmbZIP20.20这样的基因的调节.
结论:
- ZmEREB167可以负面调节玉米内的发育.
- ZmEREB167通过抑制目标基因,影响粉积累和粒大小.
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