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相关概念视频

Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
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Updated: May 30, 2025

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
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细胞READR:一个基于ADAR的RNA传感器-执行器设备.

Xiaolu Yang1, Kehali Woldemichael1, Xiao Guo2

  • 1Department of Neurobiology, Duke University School of Medicine, Durham, NC, United States.

Methods in enzymology
|January 27, 2025
PubMed
概括
此摘要是机器生成的。

细胞READR是一种新的RNA传感器技术,可以检测特定的细胞RNA来控制蛋白质的产生. 这种可编程系统可以监控和操纵各种物种和组织中的细胞.

关键词:
阿达尔 (ADAR) 是一个叫做ADAR的词.细胞类型 细胞类型可以编程的可编程.在RNA驱动器执行器.编辑RNA的RNA编辑这是一个RNA传感器RNA传感器.

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科学领域:

  • 分子生物学分子生物学
  • 生物技术是生物技术.
  • 遗传学 是一个遗传学.

背景情况:

  • RNAs对于基因调节和细胞分化至关重要.
  • 感知和响应活细胞中的RNA配置文件对于生物和医学应用至关重要.
  • 现有的技术缺乏广泛的蜂监控所需的可编程性和多功能性.

研究的目的:

  • 为了介绍CellREADR,一个可编程RNA传感器-执行器技术.
  • 为了证明CellREADR能够将RNA检测与效应蛋白翻译结合起来.
  • 为在细胞培养和动物模型中实施CellREADR提供详细的协议.

主要方法:

  • 细胞READR利用一种在RNA (ADAR) 中介的A-to-I编辑机制上起作用的腺脱氨酶.
  • 该系统包括一个5'传感器区域,与目标RNA和3'蛋白质有效载荷相补充.
  • 有效载荷翻译由RNA结合激活,通过ADAR编辑删除一个停止编码子.

主要成果:

  • 在哺乳动物细胞培养物中成功设计,克隆和验证了CellREADR.
  • 该技术证明了基于RNA检测的细胞活动的有效监测和操纵.
  • 通过在脑组织中使用基于AAV的输送建立了体内实现.

结论:

  • 细胞READR代表了新一代可编程RNA设备,用于监测和操纵动物细胞.
  • 这项技术简单,多功能,并且可以在各种组织和物种中通用.
  • 详细的协议有助于在各种实验环境中实现CellREADR.