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在卵细胞在体外成熟过程中激活蛋白质分解

Filiz Tepekoy^1,2,3, Berk Bulut⁴, Erdal Karaoz⁵

¹Department of Histology and Embryology, Faculty of Medicine, Altinbas University, Istanbul, Turkey.

Molecular reproduction and development

|January 28, 2025

概括

像PIP2和PMA这样的质子激活剂可以通过增强 lysosomal 活性和蛋白质分解激活来改善老牛卵细胞的体外成熟 (IVM). 在特定的度和时间范围内观察到最优的结果,这表明对卵子质量的时间和剂量依赖的影响.

科学领域:

生殖生物学生殖生物学
细胞动力学细胞动力学
辅助生殖技术 (ART) 是一种辅助生殖技术.

背景情况:

在体外成熟 (IVM) 的成功率随着牛卵细胞的年龄而下降.
实现蛋白质稳定对于改善老化卵细胞的受精潜力至关重要.
需要针对细胞动态的新方法来增强IVM结果.

研究的目的:

为了研究质子激活剂 (PIP2,PMA,DOG) 对老牛卵细胞蛋白质静止的作用.
为了确定IVM期间溶酶体激活的最佳度和时间点.
评估这些激活剂对卵细胞形态,蛋白质溶解激活和蛋白质炭基化的影响.

主要方法:

年龄较大的牛卵细胞 (13-16岁) 接受了IVM,其度不同,包括PIP2,PMA和DOG.
使用DQ-Red BSA和Lysotracker探针评估了溶酶体激活.
分析了卵细胞形态,直径,极体挤出 (PBE) 和蛋白质碳化 (oxyblot).

主要成果:

PIP2和PMA对卵细胞质量和蛋白质活性产生了积极的影响,特别是在更短的IVM持续时间 (6小时) 时.
特定度的PIP2和PMA保持卵细胞得分接近控制水平在较晚的时间点 (12小时,18小时).
溶解体密度在PIP2和PMA治疗时增加,而蛋白质碳化在PIP2 (0.1μM) 最低.

结论:

质子激活剂PIP2和PMA证明对牛卵细胞IVM产生积极影响.
PIP2和PMA的有效性取决于度和潜伏时间.
这些发现提供了改善老化卵细胞IVM成功率的潜在策略.

关键词:

狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗 IVM IVM IVM IVM IVM IVM IVM IVM IVM IVM IVM IVM IVM IVM IVM 在PIP2中,PIP2是PIP2.在PMA中,PMA是PMA.lysosome 溶解酶体是如何形成的卵子细胞卵子细胞

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