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在糖尿病心肌病中构建一个潜在的功能性长非编码RNA-microRNA-mRNA网络

Qiwen Cao¹, Zhihui Dong², Yangbo Xi²

¹Department of Endocrinology, Bin Hai Wan Central Hospital of Dongguan, Dongguan, China.

Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences

|January 28, 2025

概括

糖尿病心肌病 (DCM) 与2型糖尿病有关,增加了心力衰竭风险. 这项研究建立了一个竞争的内源RNA (ceRNA) 网络,以确定DCM的潜在生物标志物和治疗点.

科学领域:

心血管生物学心血管生物学
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

糖尿病心肌病 (DCM) 是2型糖尿病的严重并发症,增加心力衰竭风险和死亡率.
目前对DCM的治疗方法有限,尽管存在各种相关机制.
了解DCM的分子基础对于开发有效疗法至关重要.

研究的目的:

在糖尿病心肌病 (DCM) 中构建一个全面的竞争性内源性RNA (ceRNA) 网络.
确定DCM中差异表达基因 (DEG) 和关键调控相互作用.
发现DCM潜在的诊断生物标志物和治疗点.

主要方法:

整合了三个基因表达数据集 (GSE161827,GSE161931,GSE241166) 来识别DCM中的DEG.
执行了基因本体学,KEGG通路和GSEA用于功能丰富分析.
使用 lncRNAs,miRNAs 和 DEGs 之间的预测相互作用构建 ceRNA 网络,并通过 PPI 网络分析识别了枢纽基因.

主要成果:

确定了105个与DCM相关的DEGs (44个上调,61个下调).
在DCM中发现脂肪酸代谢和炎症反应的显著丰富.
构建了一个包含9个mRNA,17个miRNA和10个lncRNA的ceRNA网络,其中Cdh20和Cacna2d2作为枢纽基因.

结论:

已识别的ceRNA网络和枢纽基因为DCM病原体提供了洞察力.
这些发现表明DCM的潜在诊断生物标志物和治疗点.
需要进一步的实验验证和临床研究来将这些发现转化为临床实践.

关键词:

竞争的内源性RNA具有竞争性.糖尿病心肌病糖尿病心肌病长非编码RNA是什么意思它们是mRNARNA.这是一个微型RNA.

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lncRNA - Long Non-coding RNAs

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