降低NAD+通过诱导线粒体裂变抑制OLP T细胞的亡
Zhuo-Yu Zhang1, Fang Wang1,2, Gang Zhou1,3
1State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, People's Republic of China.
Journal of inflammation research
|January 28, 2025
概括
口腔平状体T细胞中尼古丁胺胺氨基二核酸 (NAD+) 水平降低会促进线粒体裂变并降低亡. 补充NAD+可能会恢复OLP中的T细胞功能.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 线粒体动力学的动力学
背景情况:
- 口腔平坦 (OLP) 是一种慢性T细胞介导的炎症性疾病.
- 线粒体动力学,特别是裂变,对于T细胞的功能和命运至关重要.
- 尼古丁胺胺二核酸 (NAD+) 是线粒体重塑和细胞能量代谢的关键调节剂.
研究的目的:
- 为了研究NAD+在调节线粒体分裂和T细胞的亡中的作用,在OLP炎症环境中.
- 探索NAD+水平如何影响OLP T细胞中的线粒体结构和功能.
主要方法:
- 从OLP患者中分离T细胞和血.
- 使用电子显微镜和染色对线粒体形态的表征.
- 在T细胞中对线粒体裂变 (Drp1 knockdown) 和NAD+水平 (使用β-尼古丁胺胺 mononucleotide和FK866) 的实验性操纵.
主要成果:
- OLP T 细胞表现出线粒体分裂的增加,由Drp1的升高和Mfn2表达的降低证明,与亡的减少相关.
- 在OLP T细胞和暴露于OLP等离子体的Jurkat细胞中降低了NAD+水平,导致线粒体裂变增强,线粒体膜潜力受损,呼吸减少.
- 在暴露于OLP等离子体的Jurkat细胞中,Drp1的敲击增加了细胞亡和减少了增殖.
- 补充NAD+可以逆转这些有害影响,恢复NAD+水平,抑制裂变,促进亡.
结论:
- 在OLP T细胞中降低的NAD+水平有助于增强线粒体分裂和减少亡.
- 补充NAD+证明了作为一种治疗策略的潜力,通过调节线粒体功能来恢复OLP中的T细胞平衡.
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