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Updated: May 30, 2025

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在活细胞中探测和成像脂动态

Zhongsheng Wu1,2, Yongtao Du1,2, Tom Kirchhausen3,4,5

  • 1State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.

Life metabolism
|January 28, 2025
PubMed
概括

新的生物传感器技术和成像技术允许科学家观察活细胞中的脂分布和动态. 这有助于我们更好地理解这些分子如何维持细胞结构和功能.

关键词:
生物传感器生物传感器脂质结合域是一个脂质结合域.活细胞成像技术使用.脂酸是一种脂酸.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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