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Updated: May 30, 2025

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Accessing the Cytotoxicity and Cell Response to Biomaterials
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酸激活了外在和内在的apoptotic过程在小鼠水泥细胞
Shih-Kai Lo1, Ni-Yu Su1,2, Chun-Chuan Su1
1School of Dentistry, Chung Shan Medical University, Taichung, Taiwan.
Journal of dental sciences
|January 28, 2025
概括
酸是一种细菌副产品,在水泥细胞中诱导细胞亡. 这种细胞死亡途径由酶信号介导,为牙周炎相关的质细胞损伤提供了洞察力.
科学领域:
- 牙周病研究 牙周病研究
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 黄油酸是厌氧细菌的代谢副产品,可以损害牙周细胞.
- 关于黄油酸对水泥母细胞的影响的研究有限.
研究的目的:
- 研究暴露于不同度的甲酸盐的小鼠水泥细胞 (OCCM.30) 中的细胞死亡机制.
- 确定酸盐是否会诱导亡,并阐明涉及的信号通路.
主要方法:
- OCCM.30细胞被用甲酸盐 (0-16mM) 治疗了48小时.
- 用MTT测定来评估细胞活力.
- 细胞周期分布和细胞亡通过流动细胞计分析.
- 卡斯巴酶激活被评估使用西式涂抹.
主要成果:
- 酸盐 (≥4mM) 抑制了以剂量依赖的方式的水泥细胞活力.
- 它诱导了亡,由增加的亚G1细胞群体和早期和晚期亡细胞的检测证明.
- 酸促进了前酶-3, -8和-9的降解,并增加了分裂的酶-3, -8和-9水平.
结论:
- 酸作为细胞毒性剂,诱导质细胞的亡.
- 亡途径是通过caspase信号激活的.
- 这项研究提供了关于在牙周炎进展过程中水泥细胞损伤的机制性见解.
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