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人类牙上皮细胞中的颗粒诱导的炎症酶

Shin Wakuda^1,2, Akira Hasuike^1,3, Kyoko Fujiwara⁴

¹Department of Periodontology, Nihon University School of Dentistry, Tokyo, Japan.

Journal of dental sciences

|January 28, 2025

概括

颗粒通过增加活性氧物种 (ROS) 来激活口腔细胞中的NLRP3炎症酶. 这一发现对于理解周植入炎和开发向治疗至关重要.

科学领域:

口腔生物学口腔生物学
免疫学免疫学免疫学
生物材料科学是生物材料的科学.

背景情况:

周植入炎是一个重大的临床挑战.
颗粒在牙科植入物中很常见,可能会影响周围植入物组织.
NLRP3炎症酶与炎症反应有关.

研究的目的:

为了研究颗粒对人类口腔上皮细胞的影响.
确定核酸结合域和富含白的重复蛋白 (NLRP) 3炎症体在颗粒诱导的炎症中的作用.
探索反应性氧物种 (ROS) 在这个过程中的参与.

主要方法:

Ca9-22人类牙上皮细胞暴露在颗粒中.
用MTT测定来评估细胞活力.
量化了基因表达 (COX2,TGF-β1,NLRP1,NLRP3,CASP1,AIM2) 和IL1β的分泌.
使用ROS测试套件测量ROS的产量.

主要成果:

在所有测试度中,颗粒保持了高细胞活力 (>75%).
观察到COX2和TGF-β1mRNA表达的剂量依赖性增加.
在暴露于颗粒后,NLRP3,CASP1mRNA表达和IL1β分泌增加.
颗粒增加了ROS的产生,而N-乙-l-氨酸减轻了ROS的产生,从而降低了NLRP3和IL1β水平.

结论:

颗粒激活了口腔上皮细胞中的NLRP3炎症酶.
反应性氧物种 (ROS) 在调解颗粒诱导的NLRP3炎症酶激活中发挥着关键作用.
这些发现提供了对围部植入炎病原体的见解.

关键词:

牙植入物是一种牙植入物.牙上皮细胞牙上皮细胞在炎症中,一些细胞会发炎.炎症炎症是一种炎症.颗粒是的颗粒.

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