相关实验视频
Updated: May 30, 2025
07:01
Induction and Testing of Hypoxia in Cell Culture
Published on: August 12, 2011
84.7K
在消化系统疾病中调节病态纤维细胞功能:缺氧的作用?
Cian M Ohlendieck1,2, Carlos Matellan1,3, Mario C Manresa1,2
1Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Dublin, Ireland.
概括
纤维细胞异质性是慢性胃肠道疾病和癌症的关键. 了解缺氧如何影响肠道纤维细胞激活对于未来的治疗策略至关重要.
科学领域:
- 胃肠病学 胃肠病学
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
背景情况:
- 纤维细胞表现出异质性,并在慢性炎症疾病和癌症中的免疫反应中发挥作用.
- 虽然已知特定纤维细胞亚型 (肌纤维细胞,炎性纤维细胞,癌症相关纤维细胞) 的作用,但其激活机制尚不清楚.
- 胃肠道 (GI) 通过细胞因子和微环境因素,如营养和氧气的可用性影响纤维细胞体的特点.
研究的目的:
- 探索纤维细胞在肠道疾病中的作用.
- 为了确定调节纤维细胞功能的介质.
- 在慢性疾病背景下调查缺氧对肠道纤维细胞激活的潜在影响.
主要方法:
- 对有关纤维细胞异质性和激活的现有文献的审查.
- 对已知的细胞因子和影响纤维细胞的微环境因素的分析.
- 讨论肠道纤维细胞中缺氧和炎症信号通路 (例如NF-κB) 之间的相互作用.
主要成果:
- 纤维细胞异质性有助于胃肠道多功能性和免疫协调在胃肠道疾病和癌症.
- 互白蛋白和TNF超级家族成员参与激活消化道中的炎症和纤维状纤维细胞特性.
- 胃肠道疾病中常见的缺氧可能与炎症信号相互作用,但它对胃肠道纤维细胞的具体影响需要进一步阐明.
结论:
- 需要进一步的研究,以充分了解肠道纤维细胞激活的机制,特别是缺氧的作用.
- 阐明缺氧-纤维细胞-炎症轴可能会揭示慢性胃肠道疾病的新型治疗点.
- 纤维细胞异质性和微环境因素是 GI 疾病病原发生的关键因素.
相关概念视频
01:24Regulation of Angiogenesis and Blood Supply
2.5K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.5K
01:09Introduction to Fibroblasts
3.0K
Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...
3.0K
01:23Hypoxia
869
Hypoxia is a medical condition characterized by an inadequate oxygen supply to body tissues. It typically manifests as a bluish discoloration of the skin and mucosae, especially in fair-skinned individuals, when hemoglobin (Hb) saturation drops below 75%.
Types of Hypoxia
There are four primary types of hypoxia, each resulting from a different cause:
1. Anemic hypoxia: This type occurs due to insufficient oxygen delivery caused by a lack of red blood cells (RBCs) or RBCs with abnormal or...
Types of Hypoxia
There are four primary types of hypoxia, each resulting from a different cause:
1. Anemic hypoxia: This type occurs due to insufficient oxygen delivery caused by a lack of red blood cells (RBCs) or RBCs with abnormal or...
869
01:22Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
2.2K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.2K
01:12Role Of Notch Signalling In Intestinal Stem Cell Renewal
2.1K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.1K
02:17The Tumor Microenvironment
6.5K
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
6.5K