末油蛋糕的生物转化用于从固态发酵提取物中生产脂酶,优化和直接固定
Bhim Singh1, Asim Kumar Jana1, Mithu Maiti Jana2
1Department of Biotechnology, Dr. B R Ambedkar National Institute of Technology Jalandhar, Jalandhar, Punjab, India.
Preparative biochemistry & biotechnology
|January 28, 2025
概括
这项研究利用农业残留物优化了真菌脂酶的产生,实现了高酶活性. 该酶在磁纳米颗粒上成功固定,为工业应用提供了具有成本效益的生物催化剂.
科学领域:
- 生物技术是生物技术.
- 酶工程是什么? 酶工程是什么?
- 工业微生物学 工业微生物学
背景情况:
- 菌脂酶是有价值的工业生物催化剂,但它们的高生产成本阻碍了广泛的商业使用.
- 使用低成本的农业残留物作为基质可以显著降低脂酶生产成本.
- 关键的挑战包括确保农业残留物和真菌物种之间的兼容性,有效的脂酶恢复和酶稳定性.
研究的目的:
- 使用固态发酵优化从选定的真菌菌株和当地可用的蔬菜油蛋糕中脂酶的生产.
- 直接将产生的脂酶固定在功能化的磁纳米粒子上.
- 评估农业残留物在具有成本效益的脂酶生产和工业应用方面的潜力.
主要方法:
- 固体发酵 (SSF) 使用*Rhizopus oryzae*和子油蛋糕.
- 使用Plackett-Burman设计和中央复合设计 (CCD) 优化脂酶生产参数.
- 直接固定原始脂酶到功能化磁纳米粒子 (MNP) 上.
主要成果:
- 在优化条件下实现了25.08U/gds的最高酶活性:2.11% (w/w) 尿素,1.18% (v/w) inokulum大小和69.99% (w/w) 含水量.
- 对MNP的固定导致蛋白质负载为68.88±3.54μg/mg,活动恢复为60.33±3.03%.
- 优化过程证明了使用农业残留物用于脂酶生产的可行性.
结论:
- 农业残留物,特别是子油蛋糕,可以有效地用于经济高效的真菌脂酶生产.
- 直接将脂酶固定在功能化磁纳米颗粒上是一种可行的酶恢复和再利用策略.
- 这种方法为工业脂酶应用提供了可持续和经济的替代方案.
更多相关视频
相关概念视频
01:25Lipid Catabolism
1.4K
Triglycerides serve as crucial long-term energy storage molecules in microorganisms, providing a dense source of metabolic energy. Their breakdown is mediated by lipases, which hydrolyze triglycerides into glycerol and free fatty acids. Each of these components follows distinct metabolic pathways, ultimately contributing to ATP synthesis and cellular energy homeostasis.Glycerol MetabolismGlycerol, released from triglyceride hydrolysis, is phosphorylated by glycerol kinase to form...
1.4K
01:27Upstream Processing
97
Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
97
01:25Production of Organic Acids
105
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
105
01:27Production of Antibiotics
265
Penicillin, one of the earliest and most widely used antibiotics, is produced industrially by the filamentous fungus Penicillium chrysogenum. Large stirred-tank bioreactors ranging from tens to hundreds of thousands of liters maintain tightly controlled temperature, pH, and dissolved oxygen conditions to support fungal metabolism and maximize antibiotic yield. Penicillin is a secondary metabolite, synthesized primarily during the stationary growth phase, which requires a carefully managed...
265
01:30Production of Pharmaceuticals
94
Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under...
94
01:25Biofuels
107
The microbial conversion of organic matter into biofuels holds potential as a renewable energy source. Among biofuel sources, microalgae are recognized as a highly efficient and adaptable feedstock for biodiesel production, owing to their rapid biomass accumulation, elevated lipid productivity, and capacity to proliferate in diverse aquatic systems, including freshwater, marine, and wastewater habitats. Unlike terrestrial crops, microalgae do not compete for land and can achieve significantly...
107