相关实验视频

Updated: May 30, 2025

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

Published on: June 16, 2021

孕激素通过两个具有PLA2活性的有约束性共受体诱导半变异

Nancy Nader^1,2, Lama Assaf^1,3, Lubna Zarif¹

¹Calcium Signaling Group, Research Department, Weill Cornell Medicine Qatar, Education City, Qatar Foundation, Doha, Qatar.

|January 28, 2025

概括

孕激素 (P4) 通过膜受体 (mPRs) 快速触发卵细胞成熟. 这项研究确定ABHD2是具有脂酶A2活性的共同受体,对P4至关重要.

科学领域:

内分泌学在内分泌学.
分子生物学分子生物学
生殖生物学生殖生物学

背景情况:

孕激素 (P4) 是一种调节生殖和代谢功能的类固醇激素.
经典的P4信号涉及核受体,但通过膜P4受体 (mPRs) 进行的快速,非基因组信号理解较少.
对于非基因组P4信号的精确信号传导级联仍然难以捉摸.

研究的目的:

阐明非基因性孕激素 (P4) 信号传导的信号传导级联.
确定参与P4介导的Xenopus卵细胞成熟的关键分子参与者.
描述ABHD2在通过mPRs传递P4信号中的作用.

主要方法:

利用Xenopus卵细胞成熟作为一个生理模型.
采用功能测定和无偏见/有针对性的基于细胞的脂质组学.
研究了ABHD2与mPRβ结合的脂酶A2 (PLA2) 活性.

主要成果:

确定了脂质酸酶ABHD2作为与mPRβ一起重要的共同受体,用于触发Xenopus卵细胞成熟.
证明ABHD2具有mPRβ依赖的脂酶A2 (PLA2) 活性.
表明P4信号通过ABHD2-介导的PLA2活性进行分叉,诱导mPRβ内细胞分裂并产生G蛋白结合受体激动剂.

结论:

孕激素 (P4) 通过一种非基因的途径驱动卵细胞成熟,需要mPRβ和ABHD2作为共同受体.
而ABHD2的PLA2活性对于启动P4介导的半分裂至关重要.
这种机制涉及mPRβ细胞内和生成激活G蛋白结合受体的脂质信使.

关键词:

这就是ABHD2的ABHD2.在PLA2中,PLA2是PLA2.细胞生物学细胞生物学脂质的信使脂质的信使膜孕激素受体β受体是什么非基因组信号传输孕激素是孕激素的一种.异国主义者 (Xenopus) 是

更多相关视频

Xenopus laevis as a Model to Identify Translation Impairment

Xenopus laevis as a Model to Identify Translation Impairment

Published on: September 27, 2015

Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes

Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes

Published on: May 11, 2017

相关概念视频

Meiosis II

Meiosis II

Meiosis II entails cell division and segregation of the sister chromatids, resulting in the production of four unique haploid gametes. The steps for meiosis II are similar to mitosis, except that meiosis II occurs in haploid cells, whereas mitosis occurs in diploid cells.
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...

Gonadal and Placental Hormones

Gonadal and Placental Hormones

The gonads, namely the testes in males and the ovaries in females, are pivotal in producing gonadal hormones that orchestrate the intricate processes of sexual development and reproduction.
In males, testosterone is the primary gonadal androgen. It plays a central role in the maturation of male reproductive organs — the penis and testes. Additionally, testosterone is instrumental in the development of secondary sexual characteristics — a deep voice as well as facial and pubic hair...

Ovarian Cycle

Ovarian Cycle

The menstrual cycle includes a critical component known as the ovarian cycle, which undergoes two main phases each month—the follicular phase and the luteal phase. The follicular phase is variable and averaging around 14 days. Ovulation, triggered by a surge in luteinizing hormone (LH), marks the transition between the two phases. The second phase, the luteal phase, is relatively consistent, lasting approximately 14 days, and is marked by the activity of the corpus luteum. While a cycle...

IP3/DAG Signaling Pathway

IP3/DAG Signaling Pathway

Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and...

Feedback Regulation of Calcium Concentration

Feedback Regulation of Calcium Concentration

Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...

Meiosis I

Meiosis I

Meiosis is a carefully orchestrated set of cell divisions, the goal of which—in humans—is to produce haploid sperm or eggs, each containing half the number of chromosomes present in somatic cells elsewhere in the body. Meiosis I is the first such division, and involves several key steps, among them: condensation of replicated chromosomes in diploid cells; the pairing of homologous chromosomes and their exchange of information; and finally, the separation of homologous chromosomes by...