CD40连接体和CD40:表达,调节和功能在母婴接口在猪
概括
CD40联体-CD40信号系统对于确定猪怀孕至关重要. 这个系统通过在怀孕早期影响子宫内膜内皮细胞功能来调节母亲的免疫力.
科学领域:
- 生殖免疫学 生殖免疫学
- 细胞和分子生物学是细胞和分子生物学.
背景情况:
- 在猪中怀孕的建立需要一个平衡的母婴免疫环境.
- 集群分化连接体40 (CD40L) 和它的受体CD40在怀孕早期在猪子宫内表达.
- 猪怀孕中CD40L-CD40信号传导的具体作用尚未完全理解.
研究的目的:
- 调查猪在母婴接口的CD40L-CD40系统的表达,调节和功能.
- 确定CD40L-CD40信号在怀孕早期对子宫内膜内皮细胞的影响.
主要方法:
- 转录组学分析以确定CD40L和CD40表达.
- 免疫组织化学用于在子宫内膜组织中定位CD40L和CD40蛋白质.
- 使用培养的猪子宫内皮细胞 (pENDO) 进行体外研究,以评估CD40L功能.
- 用干扰素对子宫内膜外开体进行治疗,以研究调节效应.
主要成果:
- CD40L和CD40mRNA水平在妊娠15日的子宫内膜达到峰值.
- CD40L蛋白定位在光上皮细胞中,而CD40在上皮细胞,树皮细胞和内皮细胞中发现.
- 在怀孕早期,Conceptus表达了CD40,CD40L和CD40都存在于晚期.
- 在子宫内膜扩展体中,干扰素-高调的CD40L和CD40表达.
- CD40L促进了pENDO细胞迁移和改变了参与血管生成,粘附,化学因子和免疫的基因表达.
结论:
- 在猪中,CD40L-CD40系统在母婴接口表达和功能.
- 这种信号通路可能在调节子宫内皮细胞功能方面发挥着重要作用.
- CD40L-CD40信号通过调节子宫免疫环境和内皮细胞行为,有助于怀孕的建立.
相关概念视频
Transcytosis of IgG
2.7K
Transcytosis is the process in which molecules are internalized by endocytosis, transported across the cell, and released through exocytosis from the opposite end of the cell. Molecules such as insulin, immunoglobulins, and certain nutrients are transferred through the recycling endosomes by recycling and transcytosis.
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
2.7K
Hedgehog Signaling Pathway
7.3K
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
7.3K
Regulation of Angiogenesis and Blood Supply
2.5K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.5K


