在干部到尾部的过渡过程中,Tgfbr1调节侧板介质皮和内皮的重组
Anastasiia Lozovska1, Ana Casaca1,2, Ana Novoa1,2
1Instituto Gulbenkian de Ciência, Rua da Quinta Grande, Oeiras, Portugal.
eLife
|January 28, 2025
概括
转化生长因子β受体1 (Tgfbr1) 对于哺乳动物的干到尾的过渡至关重要. Tgfbr1调节侧板半皮和内皮重塑,对后肢和外部生殖器发育至关重要.
科学领域:
- 发展生物学 发展生物学
- 分子生物学分子生物学
- 胚胎发生是胚胎发生.
背景情况:
- 干到尾的过渡是哺乳动物胚胎发育的关键阶段.
- 这种过渡涉及形成关键结构,如肠道和泌尿器官出口,后肢和外部生殖器.
- 它还标志着轴延伸驱动器从表皮质/原始链条 (PS) 转移到尾芽.
研究的目的:
- 为了研究转化生长因子β受体1 (Tgfbr1) 在哺乳动物干到尾部过渡中的作用.
- 阐明Tgfbr1在这种过渡过程中侧板半皮体 (LPM) 和胚胎内皮体重塑中的功能.
- 在LPM衍生组织中识别Tgfbr1的下游作用因子.
主要方法:
- 对具有Tgfbr1突变的小鼠胚胎进行分析.
- 检查Tgfbr1突变体中的LPM和胚胎内皮发育.
- 血管改造的调查,包括背部大动脉和动脉.
- 评估Isl1无突变物以确定下游Tgfbr1效应物.
主要成果:
- Tgfbr1信号传递对于LPM层的融合以及后肢和外生殖器原始的形成至关重要.
- 缺少Tgfbr1会破坏血管重塑,影响胚胎和胚胎外循环之间的联系.
- Tgfbr1控制了胚胎内皮的扩张,形成水和尾肠.
- 在LPM衍生的组织中,Isl1作用于Tgfbr1的下游,在零突变者中显示类似的LPM和血管缺陷.
- 在回归后,Tgfbr1调节后部PS命运,影响LPM和内皮重塑.
结论:
- Tgfbr1是干到尾部过渡的关键调节者,控制LPM和内皮重塑.
- Tgfbr1信号传递对于建立后部身体规划至关重要,包括后肢和外部生殖器.
- Tgfbr1处于监管级联的顶部,控制着这一必不可少的发育过程.
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