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Methods of Sterilization II: Chemical Methods01:30

Methods of Sterilization II: Chemical Methods

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In healthcare, the chemical method of sterilization uses chemical sterilants to treat surgical instruments and medical supplies to help prevent the transmission of infectious pathogens to patients. Due to heat sensitivity, most medical supplies and equipment should not be exposed to high temperatures. These parts include rubber, plastic, glass, and other similar elements.
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
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Transmission-based Precautions II: Airborne and Protective Environment01:25

Transmission-based Precautions II: Airborne and Protective Environment

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Transmission-based precautions are for patients infected or suspected to be infected (or colonized) with organisms posing a significant risk to others. The transmission precautions include airborne and protective environment precautions.
Airborne precautions:
Use airborne precautions when treating patients known or suspected to have diseases that spread through the air—for example, tuberculosis or measles. These organisms are present in smaller droplets expelled by an infected person and...
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Updated: May 30, 2025

Author Spotlight: Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies
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Author Spotlight: Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies

Published on: March 17, 2023

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使用臭氧气的生物安全柜的转换方法.

Mitsuru Mizuno1, Daisuke Suda2, Chima Matsumura2

  • 1Center for Stem Cell and Regenerative Medicine, Institute of Science Tokyo, Bunkyo-ku, Tokyo, Japan.

PloS one
|January 28, 2025
PubMed
概括

生物安全柜中的臭氧发生器有效地减少细菌,包括耐药性内分泌体和内毒素. 这项技术增强了细胞产品制造的无菌环境,确保了更安全和可重复的生产转换.

科学领域:

  • 微生物学 微生物学
  • 生物技术是生物技术.
  • 环境科学 环境科学

背景情况:

  • 维持无菌条件在细胞产品制造中至关重要,因为无法消毒活细胞产品.
  • 来自人体组织的原材料往往含有细菌,需要在生产批次之间进行有效的环境净化.
  • 传统的清洁方法与高度耐药的污染物如细菌内和内毒素作斗争.

研究的目的:

  • 评估配备臭氧发生器的生物安全柜的有效性,用于环境净化.
  • 评估设备对具有挑战性的微生物的有效性,包括内毒素,内和真菌.
  • 确定臭氧处理对简化电池制造业生产转换的潜力.

主要方法:

  • 该研究使用了一个带有集成臭氧发生器的生物安全柜.
  • 测试了特定的细菌菌株:Pseudomonas aeruginosa (内毒素生产者),Bacillus subtilis (内原体) 和Aspergillus brasiliensis (形成子的真菌).
  • 与对照组相比,测量了臭氧暴露后的殖民地形成单位 (CFU) 和内毒素水平.

主要成果:

  • 臭氧处理显著降低了Pseudomonas aeruginosa CFU和内毒素水平.
  • 观察到Bacillus subtilis内和Aspergillus brasiliensis子形成的明显减少.

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  • 臭氧发生器证明了对抗消毒剂耐药细菌,内和内毒素的有效性.

    • 结论:

    • 产生臭氧的生物安全柜在A级环境中显示出对抗细菌的巨大潜力.
    • 该设备提供了一种简化,可重复的脱污方法,可能减少操作员的压力.
    • 需要进一步验证,但该技术可以提高细胞产品制造中的安全性.