生物医学应用的聚合物架构的多光子写入和读取新兴技术
Jieliyue Sun1, Sixian Jia2, Chenhui Shao2
1School of Engineering, Brown University, Providence, Rhode Island, USA;
Annual review of biomedical engineering
|January 28, 2025
概括
双光子聚合 (TPP) 为生物医学应用提供了先进的3D纳米制造. 这次审查涵盖了TPP.
科学领域:
- 增材制造 增材制造 增材制造
- 生物医学工程 生物医学工程
- 材料科学 材料科学 材料科学
背景情况:
- 双光子聚合 (TPP) 是一种增材制造技术,在科学和工程领域日益重要.
- TPP提供了独特的3D,纳米尺度的自由形式制造,使其适合复杂的生物医学应用.
- 通过商业系统增加的可访问性加速了TPP在研究和开发中的采用.
研究的目的:
- 审查通过双光子聚合实现的多样化的生物医学应用.
- 介绍 3D TPP 制造结构的模式和特征方法的当前状态.
- 探索机器学习在TPP过程控制中的作用,并讨论未来的机会.
主要方法:
- 对生物医学领域的双光子聚合现有文献的综述.
- 分析当前用于制造和表征3DTPP结构的技术.
- 讨论用于优化TPP流程的机器学习应用程序.
主要成果:
- 由于其高分辨率和3D制造能力,TPP能够实现广泛的生物医学应用.
- 描述方法 (in situ和ex situ) 对于确保TPP制造结构的忠实性至关重要.
- 机器学习显示了增强TPP过程控制和效率的前景.
结论:
- 双光子聚合是一种强大的技术,用于先进的生物医学应用.
- 进一步发展模式,阅读和机器学习的整合将推动未来的创新.
- 在不断变化的生物医学工程领域,TPP面临重大挑战和机遇.
相关概念视频
01:18Imaging Biological Samples with Optical Microscopy
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
01:37Super-resolution Fluorescence Microscopy
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
01:07Electron Microscope Tomography and Single-particle Reconstruction
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...