相关实验视频

Updated: May 30, 2025

Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase

Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase

Published on: November 8, 2019

基因工程电离蛋白纳米基纳米酶组装用于细胞内超氧化物清理

Qiqi Liu^1,2, Zhanxia Gao¹, Xiangyun Zhang²

¹School of Medicine, Nankai University, Tianjin, 300071, China.

Nature communications

|January 28, 2025

概括

经过基因工程改造的电离性费里丁纳米 (iFTn) 能够逃离内溶酶体,以清除细胞内自由基. 这些纳米酶通过调节与氧化压力相关的基因来保护心脏损伤.

科学领域:

生物材料科学生物材料科学
纳米技术纳米技术
生物化学生物化学

背景情况:

纳米酶对于减轻氧化应激至关重要,但由于内溶性质诱捕,它们在细胞内自由基清理方面面临挑战.
开发用于纳米酶输送到细胞质中的策略对于提高其治疗疗效至关重要.

研究的目的:

设计可离子化费里纳米 (iFTn),能够通过内溶酶体逃逸,进行细胞质输送.
创建基于iFTn的级联纳米酶,具有增强的超氧化物转化酶和酶模仿活动.
评估这些纳米酶在保护心脏氧化损伤方面的 in vivo 疗效.

主要方法:

人类重链费里丁的基因工程与可电离的胺-胺-胺酸 (9H2E) 序列.
使用聚乙烯糖醇 (PEG) 组装iFTn纳米结构以形成类似链的结构.
纳米酶活动的体外表征和心脏缺血-再输液 (IR) 的小鼠模型中的体内评估.
对IR受损的心脏组织进行RNA测序分析,以确定调制的基因通路.

主要成果:

成功生成了具有内溶酶体逃脱能力的iFTn.
开发了四层级级级纳米酶,表现出高超氧化物脱酶和酶模仿活动.
在体内证明了对心脏氧化损伤的显著保护.
RNA测序揭示了与超氧化离子,过氧化和线粒体功能相关的基因的纳米酶介导调制.

结论:

经过基因工程改造的iFTn有效地克服了细胞质输送的内溶酶体捕获.
基于iFTn的级联纳米酶显示出对与氧化压力相关的疾病,特别是心脏损伤的治疗药物有前途.
这些可电离蛋白纳米载体为开发先进的药物输送系统提供了多功能平台.

更多相关视频

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform

Published on: November 7, 2013

Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization

Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization

Published on: January 24, 2025

相关概念视频

Electron Transport Chain: Complex III and IV

Electron Transport Chain: Complex III and IV

During the electron transport chain, electrons from NADH and FADH2 are first transferred to complexes I and II, respectively. These two complexes then transfer the electrons to ubiquinol, which carries them further to complex III. Complex III passes the electrons across the intermembrane space to Cyt c, which carries them further to complex IV. Complex IV donates electrons to oxygen and reduces it to water. As electrons pass through complexes I, III, and IV, the energy released aids the pumping...

The Supercomplexes in the Crista Membrane

The Supercomplexes in the Crista Membrane

The mitochondrial cristae membrane is the primary site for the oxidative phosphorylation (OXPHOS) process of energy conversion mediated through respiratory complexes I to V. These complexes have been widely studied for decades, and it has been proven that they form supramolecular structures called respiratory supercomplexes (SC). These higher-order complexes may be crucial in maintaining the biochemical structure and improving the physiological activity of the individual complexes while...

The Electron Transport Chain

The Electron Transport Chain

The electron transport chain or oxidative phosphorylation is an exothermic process in which free energy released during electron transfer reactions is coupled to ATP synthesis. This process is a significant source of energy in aerobic cells, and therefore inhibitors of the electron transport chain can be detrimental to the cell's metabolic processes.
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...

Electron Transport Chain: Complex I and II

Electron Transport Chain: Complex I and II

The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...