由蛋白酸酶4介导的多层调节网络控制了Magnaporthe oryzae中的碳催化剂抑制和去抑制
Zhicheng Huang1, Qing Wang1, Yan Li1
1Xianghu Laboratory, College of Life Sciences, Zhejiang University, Hangzhou, China.
Communications biology
|January 28, 2025
概括
这项研究揭示了Pp4c,一种蛋白质酸酶,是真菌生长和致病性的关键. 它与Snf1激酶和调节器CreA/Crf1一起工作,以控制Magnaporthe oryzae中的碳源利用.
科学领域:
- 分子生物学分子生物学
- 菌类学 菌类学是指菌类学.
- 生物化学 生物化学
背景情况:
- 碳催化剂抑制 (CCR) 和去抑制 (CCDR) 对真菌病原性至关重要.
- 致病真菌中CCR/CCDR的调节机制尚未完全理解.
研究的目的:
- 调查蛋白质酸酶4催化子单元 (Pp4c) 在Magnaporthe oryzae中的作用.
- 阐明管理碳源利用和致病性的监管网络.
主要方法:
- 在M. oryzae.中识别Pp4c.
- 蛋白质相互作用和酸化的分析.
- 研究Pp4c,Smek1,Snf1,CreA和Crf1在碳代谢中的作用.
主要成果:
- Pp4c对于M. oryzae的生长,结合,毒性和营养利用是必不可少的.
- 蛋白质酸酶4复合体,Snf1激酶和转录因子CreA/Crf1共同调节碳源利用.
- 在富含葡萄糖的条件下,Snf1和Smek1直接调节CreA/Crf1酸化.
- 在富含L-氨酸的条件下,Snf1通过Pp4c和Smek1间接调节CreA/Crf1脱化.
结论:
- 该网络对CreA和Crf1的酸化驱动CCR和CCDR,影响植物细胞壁碳代谢.
- 这种调节途径对于植物病原菌的真菌病原性至关重要.
- 结果提供了关于碳代谢和致病性的CCR/CCDR调节的见解.
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