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一个bHLH转录因子RrUNE12调节盐分耐受性,促进酸盐合成

Zhenying Yang¹, Ling Lin¹, Min Lu¹

¹Engineering Research Center of National Forestry and Grassland Administration for Rosa Roxburghii, Agricultural College, Guizhou University, Guiyang, 550025, People's Republic of China.

Plant cell reports

|January 28, 2025

概括

一种新型的转录因子RrUNE12通过与RrGGP2促进体结合,激活水果中的L-甲酸 (AsA) 生物合成. 这通过调节抗氧化基因并维持ROS恒温来增强植物对盐应激的耐受性.

科学领域:

植物分子生物学植物分子生物学
植物生理学植物生理学
生物化学生物化学

背景情况:

酸 (L-ascorbic acid,AsA) 是植物中重要的抗氧化剂,对应激防御至关重要.
GDP-L-银酸盐酸酶2 (RrGGP2) 是Rosa roxburghii*水果中AsA过度产生的关键基因.
在非生物性压力下*RrGGP2*的转录调节尚未完全理解.

研究的目的:

确定和描述调节RrGGP2和AsA生物合成的转录因子在Rosa roxburghii水果中的作用.
为了研究RRUNE12在应对盐应激时的功能.

主要方法:

通过与*RrGGP2*和AsA水平的相关性分析,识别了bHLH转录因子RrUNE12 .
对 RrUNE12.12 的核定位和促进剂结合试验.
在R. roxburghii*水果和中,基因过度表达和沉默.
用外源性NaCl进行压力治疗.
在番茄植物中,RrUNE12的稳定过度表达.

主要成果:

RrUNE12直接与*RrGGP2*促进体结合,增强其转录,从而增加ASA水平.
盐应激显著诱导RrUNE12的表达.
在R. roxburghii和番茄中RrUNE12的过度表达通过调节抗氧化剂相关基因并维持ROS平衡来增加对盐应激的耐受性.
RrUNE12过度表达导致AsA含量和抗氧化能力更高.

结论:

RrUNE12作为Rosa roxburghii水果中AsA生物合成的积极调节剂.
RrUNE12通过提高ASA水平和增强氧化抵抗力,在减轻盐应激方面发挥着重要作用.
RrUNE12是通过基因工程改善作物应激耐受性的有希望的目标.

关键词:

罗莎·罗克斯堡的条约.在 RrGGP2 里面.第1212章没有了 L-阿斯科布酸 (AsA) 是一种盐的压力是盐的压力.

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