使用扫描电子显微镜在内牙治疗期间去除涂抹层的效率的评估:一个体外研究研究
Svetlana Razumova1, Anzhela Brago1, Anzhelika Kryuchkova1
1Department of Propedeutics of Dental Diseases, Medical Institute, Peoples' Friendship University of Russia (RUDN University), 6 Miklukho-Maklaya Street, Moscow, 117198, Russian Federation.
BMC oral health
|January 28, 2025
概括
与传统的乙烯基胺三酸 (EDTA) 灌相比,乙烯基酸溶液显著改善了根管中涂抹层的去除. 这种增强的清洁促进了更好的内牙治疗结果.
科学领域:
- 牙周内科医院 牙周内科医院 牙周内科医院
- 牙科材料科学 牙科材料科学
背景情况:
- 涂抹层复杂化了根管灌,可能会影响内牙治疗的成功.
- 有效地去除涂抹层对于可预测的治疗结果至关重要.
研究的目的:
- 为了比较不同灌方案的有效性,用于涂抹层和根通道中的碎片清除.
- 使用扫描电子显微镜 (SEM) 来定量评估涂抹层的去除.
主要方法:
- 30个完整的拔牙被分为三个灌组.
- 第1组:3%的二 (NaOCL) +17%的乙烯基二胺三酸 (EDTA).
- 第二组:3%的NaOCL+9%的乙烯酸盐溶液.
- 第三组:在3%的NaOCL中含有9%的乙二德酸盐溶液.
- 使用了SEM分析和曼-惠特尼测试.
主要成果:
- 观察到涂抹层去除质量的显著差异,特别是在顶端三分之一.
- 与EDTA相比,使用9%埃迪德罗酸盐的方案显示出更好的涂抹层去除.
- 基于埃蒂德罗纳特的方案被发现比EDTA有效四倍.
结论:
- 用埃迪德罗酸盐溶液灌可以在根管中提供优质的涂抹层去除.
- 基于乙二酸盐的灌方案代表了牙周清洁疗效的有前途的进步.
相关概念视频
Scanning Electron Microscopy
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
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Fundamental Principles
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Preparation of Samples for Electron Microscopy
To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...


