对照乳母奶牛的发酵概况和微生物种群的液采集方法
1Department of Animal Science, University of Florida, Gainesville, FL 32611.
JDS communications
|January 29, 2025
概括
乳汁液收集方法影响奶牛乳汁的pH值,氨-N和挥发性脂肪酸 (VFA). 管 (RC) 和胃管 (ST) 方法产生不同的微生物配置和发酵参数.
科学领域:
- 动物科学动物科学
- 类动物营养 类动物营养
- 微生物学 微生物学
背景情况:
- 准确的液分析对于了解奶牛的健康和营养至关重要.
- 胃管 (RC) 和胃管 (ST) 是常见的采样方法,但它们对结果的影响仍在争论中.
研究的目的:
- 为了比较乳母奶牛中通过RC与ST收集的小液中的发酵概况和微生物多样性.
- 评估采样方法对皮关键参数 (如pH,挥发性脂肪酸 (VFA),氨-N和微生物组合) 的影响.
主要方法:
- 在28天的时间里,用3x3拉丁方形设计使用了三只经过小吃管的哺乳乳母牛.
- 在养后4小时采集了液,使用RC或ST.
- 分析的参数包括pH,VFA,氨-N和微生物组合 (16SrRNA测序).
主要成果:
- 与RC (6.25) 相比,ST (6.88) 的液pH值更高.
- 氨-N和总VFA与RC (15.2 mg/dL和121.8 mM) 与ST (10.6 mg/dL和95.5 mM) 相比显著更高.
- 微生物组分析显示,细菌类的丰富性更大,ST的Firmicutes较低;Chao1和Shannon指数没有受到影响.
结论:
- 液收集方法显著影响液的pH值,氨-N和总VFA度.
- 虽然个体VFA比例和多样性指数 (Chao1,Shannon) 类似,但酸与酸的比率倾向于随着ST增加.
- 采样方法的选择 (RC与ST) 影响了黄发酵的关键参数和微生物群落结构.
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