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基芯片:一种微生理系统,用于培养具有改善基差异化的人类介质干细胞

Isisdoris Rodrigues de Souza¹, Andreia Akemi Suzukawa¹, Cintia Delai da Silva Horinouchi^1,2

¹Laboratório de Biologia Básica de Células-Tronco, FIOCRUZ, Rua Professor Algacyr Munhoz Mader, 3775, Instituto Carlos Chagas, Curitiba, Paraná PR 81350-010 Brazil.

In vitro models

|January 29, 2025

概括

这项研究开发了一个器官在芯片上的系统,使用人类脂肪干细胞来改善对脂肪组织的理解. 该系统增强了细胞的增殖和分化,为代谢研究提供了更好的模型.

科学领域:

生物技术是生物技术.
干细胞生物学干细胞生物学
组织工程是组织工程.

背景情况:

肥胖及其并发症是全球主要的健康问题,脂肪组织在代谢疾病中发挥着中心作用.
由于依赖动物模型,对人类脂肪组织机制的理解受到限制.
器官在芯片 (OoC) 技术为研究人类生理学和疾病提供了一个有希望的替代方案.

研究的目的:

开发和验证一个器官在芯片 (OoC) 系统,用于评估人类脂肪衍生干细胞 (hASC) 的脂肪基分化.
将OOC系统的性能与hASC培养的标准静态培养方法进行比较.
调查OOC系统在脂肪组织生物学和毒理学方面的机械研究的潜力.

主要方法:

开发一个包含hASCs的微生理系统 (MPS).
使用尼罗河红色染色对脂质滴滴进行脂肪生成差异化的评估.
通过Ki-67蛋白表达量化hASC的扩散.
使用先进的成像技术分析细胞和细胞核形态.

主要成果:

与静态96井板培养相比,Adipo-on-a-chip系统显著增强了hASC的增殖和分化.
尼罗河红色染色证实了OOC系统中脂肪滴形成的增加.
基因表达分析显示,MPS中FABP4水平较低,这表明脂质平衡发生了变化.
基-67表达表明在MPS内增加了hASC的扩散.

结论:

开发的Adipo-on-a-chip系统是研究hASC脂肪生成的可行和有效工具.
这种MPS平台增强了hASC的分化和扩散,提供了一个更具生理相关性的模型.
该系统对脂肪组织功能的机械研究和毒理学评估具有前景.

关键词:

在芯片上的脂肪组织.由脂肪酸衍生的人类介质细胞干细胞/干细胞.差异化潜力是不同的.在 Ki-67 机器人脂质的滴滴,就是脂质滴滴.微流体学微流体学在hASC的形态上,hASC的形态是:

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