相关实验视频
Updated: May 30, 2025

08:32
Conducting Multiple Imaging Modes with One Fluorescence Microscope
Published on: October 28, 2018
9.8K
基于开放框架的模块化显微镜上,开源实现了分极化解决的单射相差相对比显微镜 (pDPC)
Huihui Liu1, Sunil Kumar1,2, Edwin Garcia1,3
1LIGHT Community, Physics Department, Imperial College London SW7 2AZ, UK.
HardwareX
|January 29, 2025
概括
我们开发了一种低成本,开源的偏振差相位对比显微镜 (pDPC) 模块,用于实时相位成像. 该系统增强了对齐优化,并且很容易与光显微镜集成.
科学领域:
- 光学显微镜是一种光学显微镜.
- 阶段成像成像 阶段成像成像
- 生物物理学的生物物理.
背景情况:
- 不同相显微镜 (DPM) 能够进行定量相成像.
- 极化差分相对比显微镜 (pDPC) 提供了一个强大的,低成本的DPM单次实现.
- 现有的pDPC方法需要与特定的显微镜设置集成.
研究的目的:
- 为了展示一个新的,低成本的,开源的pDPC模块.
- 为了将pDPC与模块化开源显微镜集成",openFrame"的支架.
- 为实时阶段读取和系统对齐优化提供改进的硬件和软件.
主要方法:
- 使用极化敏感摄像头同时获得四个斜向透光图像.
- 开发了一个与"openFrame"显微镜兼容的开源pDPC模块.
- 实施了改进的硬件和软件,用于实时相位成像和系统对齐.
- 提供了背景减去和交叉调整的协议.
主要成果:
- 展示了一个低成本的,开源的pDPC模块用于定量相位成像.
- 在视野中实现实时相位读取,方便对齐优化.
- 成功地将pDPC与"openFrame"模块化显微镜支架集成在一起.
- 已验证的背景减去和交叉调整协议.
结论:
- 开发的开源pDPC模块为定量相位成像提供了具有成本效益和多功能性的解决方案.
- 实时阶段读取显著提高了系统的可用性和对齐性.
- 这种实现有助于与其他显微镜技术 (如光显微镜) 进行整合.
相关概念视频
Phase Contrast and Differential Interference Contrast Microscopy
7.4K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
7.4K
Confocal Fluorescence Microscopy
13.0K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.0K
Imaging Biological Samples with Optical Microscopy
4.6K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
4.6K

