Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

720
Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
720
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

6.4K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.4K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Proteomic Impact of Peripheral Expression of Mutant Huntingtin in <i>C. elegans</i>.

Journal of proteome research·2026
Same author

Predictive Value of the Pulmonary Artery Pulsatility Index in Pulmonary Arterial Hypertension: REVEAL Analysis.

Cardiology research·2026
Same author

Toward Metabolomics Analyses With Combined Capillary Vibrating Sharp-Edge Spray Ionization and Atmospheric Pressure Chemical Ionization.

Rapid communications in mass spectrometry : RCM·2026
Same author

iAstrocytes model cytokine influences on complement expression and neuronal network synchronization.

bioRxiv : the preprint server for biology·2026
Same author

Limited penetrance of dominantly inherited AIRE variants in a population-based cohort.

Human molecular genetics·2026
Same author

Structural Characterization of Calcium-Dependent Calmodulin-Calmidazolium Binding using Capillary Vibrating Sharp-Edge Spray-based Native Mass Spectrometry and In-Droplet Hydrogen Deuterium Exchange Mass Spectrometry.

bioRxiv : the preprint server for biology·2026

相关实验视频

Updated: May 30, 2025

Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry
07:33

Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry

Published on: October 15, 2018

14.2K

访问不同的蛋白质调整器组合与可调节的毛细血管振动尖端喷雾电离.

Daud Sharif1, Vikum K Dewasurendra2, Mst Nigar Sultana1

  • 1Department of Chemistry, West Virginia University, Morgantown, West Virginia 26506, United States.

The journal of physical chemistry. B
|January 29, 2025
PubMed
概括
此摘要是机器生成的。

毛细管振动尖端喷雾电离 (cVSSI) 控制蛋白质离子电荷状态和构造. 这项研究揭示了电压如何影响全方位离子分布,为蛋白质稳定性和质谱分析提供了洞察力.

更多相关视频

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
10:01

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Published on: November 28, 2017

19.6K
Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics
09:18

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics

Published on: April 17, 2017

9.8K

相关实验视频

Last Updated: May 30, 2025

Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry
07:33

Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry

Published on: October 15, 2018

14.2K
Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
10:01

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Published on: November 28, 2017

19.6K
Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics
09:18

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics

Published on: April 17, 2017

9.8K

科学领域:

  • 分析化学 分析化学
  • 生物化学 生物化学
  • 物理化学 物理化学

背景情况:

  • 质谱法 (MS) 对于分析蛋白质结构至关重要.
  • 在雾化微滴中控制滴滴充电会影响离子构造.
  • 毛细管振动尖端喷雾电离 (cVSSI) 提供可调节的滴滴充电.

研究的目的:

  • 为了研究使用cVSSI的电压控制滴滴充电对无处不在离子电荷状态分布 (CSD) 和形状的影响.
  • 了解滴滴动力学,电荷状态和蛋白质调整器组合之间的关系.
  • 探索cVSSI作为研究蛋白质稳定性的工具.

主要方法:

  • 利用cVSSI通过质谱仪生成和分析乌比奎离子.
  • 多种应用电压以建立不同的低电压 (LV),中电压 (MV) 和高电压 (HV) 系统.
  • 分析了每个电压模式的电荷状态分布 (CSD) 和推断的蛋白质构造.

主要成果:

  • LV 调节 (<+200 V) 显示出低电荷状态 (2+-4+) 的优势,与空气动力学分解和原生构造有关.
  • 在MV模式 (+200~+600V) 中,电荷状态 (7~12+) 更高,与滴滴加热和部分展开的物种有关.
  • 高温模式 (>+600 V) 实现了纳米电子喷射电离 (nESI) 类型的分布 (5+, 6+ 离子),表明更快的滴滴充电和本地形状采样.

结论:

  • cVSSI可以精确控制滴滴充电,影响蛋白离子构成和CSD.
  • 不同的电压模式与不同的滴滴分解路径以及由此产生的蛋白质调整器集体相关.
  • cVSSI是一种有前途的技术,用于在质谱学中研究蛋白质稳定性和 conformational动力学.