访问不同的蛋白质调整器组合与可调节的毛细血管振动尖端喷雾电离
Daud Sharif1, Vikum K Dewasurendra2, Mst Nigar Sultana1
1Department of Chemistry, West Virginia University, Morgantown, West Virginia 26506, United States.
The journal of physical chemistry. B
|January 29, 2025
概括
毛细管振动尖端喷雾电离 (cVSSI) 控制蛋白质离子电荷状态和构造. 这项研究揭示了电压如何影响全方位离子分布,为蛋白质稳定性和质谱分析提供了洞察力.
科学领域:
- 分析化学 分析化学
- 生物化学 生物化学
- 物理化学 物理化学
背景情况:
- 质谱法 (MS) 对于分析蛋白质结构至关重要.
- 在雾化微滴中控制滴滴充电会影响离子构造.
- 毛细管振动尖端喷雾电离 (cVSSI) 提供可调节的滴滴充电.
研究的目的:
- 为了研究使用cVSSI的电压控制滴滴充电对无处不在离子电荷状态分布 (CSD) 和形状的影响.
- 了解滴滴动力学,电荷状态和蛋白质调整器组合之间的关系.
- 探索cVSSI作为研究蛋白质稳定性的工具.
主要方法:
- 利用cVSSI通过质谱仪生成和分析乌比奎离子.
- 多种应用电压以建立不同的低电压 (LV),中电压 (MV) 和高电压 (HV) 系统.
- 分析了每个电压模式的电荷状态分布 (CSD) 和推断的蛋白质构造.
主要成果:
- LV 调节 (<+200 V) 显示出低电荷状态 (2+-4+) 的优势,与空气动力学分解和原生构造有关.
- 在MV模式 (+200~+600V) 中,电荷状态 (7~12+) 更高,与滴滴加热和部分展开的物种有关.
- 高温模式 (>+600 V) 实现了纳米电子喷射电离 (nESI) 类型的分布 (5+, 6+ 离子),表明更快的滴滴充电和本地形状采样.
结论:
- cVSSI可以精确控制滴滴充电,影响蛋白离子构成和CSD.
- 不同的电压模式与不同的滴滴分解路径以及由此产生的蛋白质调整器集体相关.
- cVSSI是一种有前途的技术,用于在质谱学中研究蛋白质稳定性和 conformational动力学.
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