雌激素通过GPER介导的NOTCH途径促进子宫内膜癌的扩散和迁移
Meng Qi1,2, Yuxi Jin1,2, Lulu Si1,2
1Department of Obstetrics and Gynecology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
Journal of biochemical and molecular toxicology
|January 29, 2025
概括
雌激素通过增加G蛋白结合雌激素受体 (GPER) 和激活NOTCH通路来促进子宫内膜癌 (EC) 细胞生长和转移. 抑制GPER可以逆转这些效应,突出显示GPER.
科学领域:
- 内分泌学 在内分泌学.
- 在瘤学瘤学.
- 分子生物学分子生物学
背景情况:
- 子宫内膜癌 (EC) 是一种严重的妇科恶性瘤.
- G蛋白结合雌激素受体 (GPER) 在EC进展中的作用尚未完全理解.
- 雌激素信号传递和NOTCH通路与癌症发展有关.
研究的目的:
- 调查EC中的GPER表达及其与临床病理特征的相关性.
- 评估雌激素对通过GPER调解的EC增殖和迁移的影响.
- 检查GPER在影响EC行为的NOTCH途径中的作用.
主要方法:
- 对GPER表达和临床病理特征的临床数据的分析.
- 在体外测试 (MTT,EDU,伤口愈合,transwell) 的增殖和迁移.
- 在GPER,Notch1和Hes-1蛋白水平的西部斑.
- 在体内异种移植瘤模型.
主要成果:
- 在EC中,GPER的表达上调,并与晚期临床阶段和转移有关.
- 雌激素通过对GPER,Notch1和Hes-1进行上调来增强EC细胞的增殖和迁移.
- 抑制GPER抑制了EC的扩散和迁移;NOTCH通路激活 (JAG-1) 抵消了这种抑制.
- 在体内,雌激素通过GPER介导的NOTCH通路促进EC增殖.
结论:
- GPER是雌激素驱动子宫内膜癌进展的关键调解者.
- GPER-NOTCH信号轴在EC扩散和转移中发挥着至关重要的作用.
- 针对GPER可能为子宫内膜癌提供治疗策略.
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