从Tribulus terrestris提取物中检测和描述致病性Bacillus haynesii:降低其水平的方法
Priti Darne1, Shital Palghadmal1, Nisha Mehta1
1Innovation and Drug Discovery, Sava Healthcare Limited, Research Center, MIDC, Block D1, Plot No. 17/6, Chinchwad, Pune, 411019, India.
概括
在Tribulus terrestris原料中的微生物污染被确定为Bacillus haynesii. 甲 (GA) 有效地减少了99%的微生物负载,同时保留了生物活性化合物,确保了安全的草本提取物.
科学领域:
- 微生物学 微生物学
- 药理学是指药理学,即药理学是指药理学.
- 质量控制 质量控制
背景情况:
- 由于环境因素,像Tribulus terrestris (TT) 这样的民族医学植物容易受到微生物污染.
- 评估和减轻草本原料中的微生物负载对于产品的安全性和有效性至关重要.
研究的目的:
- 在原始 Tribulus terrestris 材料中确定主要的微生物污染物.
- 评估谷氨酸甲 (GA) 和聚乙烯比古安化物 (PHMB) 在减少微生物污染方面的有效性.
- 通过有效的净化过程,确保草本提取物的安全性和质量.
主要方法:
- 使用16S rRNA测序对细菌进行隔离和鉴定.
- 生物化学测试以表征分离的细菌 (Bacillus haynesii).
- 抗生素耐药性分析和血液溶解试验.
- 用Glutaraldehyde (GA) 和多乙烯比古安化物 (PHMB) 进行治疗,以评估微生物减少.
- 高性能液体染色学 (HPLC) 检测剩余的GA.
主要成果:
- 鉴定出Bacillus haynesii是主要的细菌.
- 百菌海尼西表现出抗生素耐药性和血液溶解活性.
- 1%的甲 (GA) 实现了99%的微生物负载降低.
- GA处理没有影响提取物的产量或生物活性沙宁含量.
- 通过HPLC确认没有残留的GA,表明安全的去污过程.
结论:
- 标准化质量控制措施,包括HACCP,对于草药原料至关重要.
- 甲 (GA) 是一种有效和安全的干剂,用于清除Tribulus terrestris原料的污染.
- 确保微生物安全对于草本提取物的完整性和治疗用途至关重要.
更多相关视频
09:54Synthesis, Characterization, and Application of Superparamagnetic Iron Oxide Nanoprobes for Extrapulmonary Tuberculosis Detection
Published on: February 16, 2020
5.7K
04:52Author Spotlight: Discovering New Biopesticides from Bioactive Soil Microbe-Derived Natural Products
Published on: July 26, 2024
1.4K
相关概念视频
01:26Pulmonary Tuberculosis IV
Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
01:26Therapeutic Drug Monitoring: Drug Analysis Methods
Therapeutic Drug Monitoring (TDM) is a clinical practice that measures specific drug levels in a patient's blood or body tissues to tailor drug therapy effectively. This monitoring is critical for managing drugs with narrow therapeutic indices like digoxin and phenytoin, ensuring they are both safe and effective. For instance, monitoring theophylline levels in asthma patients involves precision and sensitivity to adjust doses according to individual responses to therapy, ensuring efficacy and...