胚胎细胞形态参数与染色体euploidy,aneuploidy和其他染色体异常之间的相关性在植入前遗传测试后 - 一个中心的回顾性研究
Alyssa Hochberg1,2, Liliane Amoura3, Xiao Yun Zhang3
1Department of Obstetrics and Gynecology, McGill University, 845 Rue Sherbrooke, O, Montreal, QC, 3HA 0G4, Canada. alyssahoch@gmail.com.
Archives of gynecology and obstetrics
|January 29, 2025
概括
胚胎细胞形态与染色体状况有关,在植入前遗传测试中检测出无细胞积分 (PGT-A). 更好的胚胎质量,扩张和内部细胞质量/肌体等级与更高的euploidy率相关,在不使用PGT-A时有助于选择.
科学领域:
- 生殖医学 生殖医学
- 遗传学 是一个遗传学.
- 胚胎学 胚胎学
背景情况:
- 在胚胎移植前进行形性遗传检测 (PGT-A) 对于体外受精 (IVF) 成功至关重要.
- 胚胎细胞形态是胚胎选择的关键因素,但其与染色体状况的相关性需要进一步调查.
研究的目的:
- 为了研究胚胎细胞形态和染色体状况之间的关联.
- 用PGT-A数据来确定形态参数是否可以预测euploidy和特定的aneuploidy类型.
主要方法:
- 通过下一代测序 (2017-2022) 进行PGT-A的169个试管婴儿周期的回顾性队列研究.
- 胚囊细胞在形态上得到了评分 (加德纳和斯科尔克拉夫特标准).
- 多变量逻辑回归分析了形态和染色体状态 (euploid,aneuploid,马赛克,复杂) 之间的关联.
主要成果:
- 在分析的826个胚胎囊中,38.86%是euploid.
- 较高的扩张,胚胎质量和托菲克托皮 (TE) /内部细胞质量 (ICM) 等级与积体的几率增加有关.
- 较高的扩张预测了单对双形状;较高的ICM等级预测了单对复杂的形状.
结论:
- 形态参数显著与胚胎细胞积体和各种积体状态有关.
- 这些发现可以帮助选择染色体上最可能的欧类母细胞囊进行移植,特别是当不进行PGT-A时.
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