细菌传感器是通过降低复杂度而进化的
Elizabet Monteagudo-Cascales1, José A Gavira2, Jiawei Xing3
1Department of Biotechnology and Environmental Protection, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, Granada 18008, Spain.
概括
细菌受体使用缓存域来感知信号. 研究人员发现,双域受体 (dCache) 中的格式识别类似于单域受体 (sCache),这表明通过简化进化.
科学领域:
- 微生物学 微生物学
- 结构生物学 结构生物学
- 生物化学 生物化学
背景情况:
- 细菌受体利用连接体结合域 (LBD) 来检测环境信号,影响细胞过程.
- 缓存域代表了细菌,古生物和真核生物中广泛存在的LBD家族,对于提取细胞质信号检测至关重要.
- 缓存域存在于单个 (sCache) 或双重 (dCache) 结构模块中,dCache域的功能不太清楚.
研究的目的:
- 研究PacF化学受体dCache域在*Pectobacterium atrosepticum*中的形式识别的功能和结构基础.
- 在格式感知中探索dCache和sCache域之间的进化关系.
- 阐明在 sCache 和 dCache 域中结合连接体的分子机制.
主要方法:
- 鉴定和描述PacF化学受体及其在化学吸引中的作用.
- 高分辨率的格式绑定的sCache和dCache域名家族成员的结构确定.
- 在不同的Cache域架构中对联结机制进行比较分析.
主要成果:
- 该PacF化学受体在其dCache域的膜距离模块上识别formate,调解化学吸引.
- 一个格式特定的sCache域的家族已经从dCache域通过失去膜近接模块进化.
- 尽管序列相同性较低,但sCache和dCache域表现出非常相似的形式结合的分子基.
结论:
- 在dCache域中的膜近接模块可能不必对连接体结合至关重要,这可能解释了它的进化损失.
- 进化的复杂性降低,例如从dCache转移到sCache域,有助于细菌受体的功能多样性.
- 这项研究增强了对细菌信号感知和塑造受体多样性的进化策略的理解.
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