相关实验视频
Updated: Jun 27, 2026

14:16
Fluorescence detection methods for microfluidic droplet platforms
Published on: December 10, 2011
22.1K
超光谱成像用于pL滴滴的高通量光学光谱
Marc Sulliger1, Jaime Ortega Arroyo1, Romain Quidant1
1Nanophotonic Systems Laboratory, Department of Mechanical and Process Engineering, ETH Zurich, 8092 Zurich, Switzerland.
Analytical chemistry
|January 29, 2025
概括
本研究介绍了一种先进的光流体平台,用于高通量滴滴分析. 它能够以前所未有的分辨率和自动化对单滴含量进行无标签查询,从而推进生物化学和生物医学查.
科学领域:
- 生物化学 生物化学
- 生物医学是生物医学.
- 微流体学 微流体学
背景情况:
- 基于滴滴的微流体提供了高通量分析,但与小体积的弱信号作斗争.
- 在高吞吐量下从picoliter滴中提取丰富的信息仍然是一个重大挑战.
研究的目的:
- 开发一个集成的光流体平台,用于对单滴含量进行高通量,无标签的分析.
- 克服信号检测的局限性,并使滴滴微流体学中的多参数分析成为可能.
主要方法:
- 集成的光流体平台结合了高光谱成像,自我引用和自动化.
- 实现了高时间和光谱分辨率,射击噪声性能有限.
- 证明了单滴含量的无标签查询.
主要成果:
- 使用高时间和光谱分辨率研究纳米粒子组成的快速动态变化.
- 使用DNA-AuNP传感器检测到到250 pM的目标DNA序列,展示传感兼容性.
- 通过自动测量,通过数小时的多重复合样本监测.
结论:
- 光流体平台弥合了高通量滴滴分析的差距.
- 显著增强滴滴微流体的内容丰富的表征能力.
- 提高生物医学和生物化学中高通量查方法的范围和有效性.
相关概念视频
Confocal Fluorescence Microscopy
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation
Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used.
There are three main types of inductively coupled plasma atomic emission spectroscopy (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used.

