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迪 (2-乙烯基) 甲酸盐通过减少精子尾巴能量供应来降低精子运动率

Xiao-Lu Li¹, Qi-Meng Li¹, Yuan-Zhuo Zheng¹

¹Department of Toxicology, School of Public Health; Suzhou Hospital of Anhui Medical University, Hefei 230032, China; Key Laboratory of Environmental Toxicology of Anhui Higher Education Institutes, Hefei 230032, China.

Ecotoxicology and environmental safety

|January 29, 2025

概括

迪 (2-乙烯基) 甲酸盐 (DEHP) 通过破坏精子尾部的能量产生来损害精子运动. 这种环境污染物减少了腺三酸盐 (ATP) 的合成,影响了精子的功能.

科学领域:

环境毒理学环境毒理学
生殖生物学生殖生物学
生物化学生物化学

背景情况:

二 (2-乙基) 甲酸盐 (DEHP) 是一种广泛存在的环境污染物.
暴露于DEHP与精子功能和发育受损有关.
德对精子尾巴结构和运动性的特定影响仍未得到充分研究.

研究的目的:

调查DEHP对精子尾巴功能和运动性的影响.
阐明DEHP影响精子能量生产的机制.
为了确定DEHP代谢物在丸毒性的作用.

主要方法:

高性能液体染色学-质谱学 (HPLC-MS/MS) 用于检测DEHP代谢物.
精子运动性分析,以量化精子运动的变化.
电子显微镜检查精子尾巴结构.
生物化学试验评估线粒体呼吸链活动和ATP合成.

主要成果:

在丸和上检测到DEHP代谢物,表明直接接触精子.
随着DEHP度的增加,精子运动率以剂量依赖的方式下降.
精子尾部没有观察到结构异常,但线粒体复合II活性和ATP合成减少.
DEHP干扰着弗拉腺因二核酸 (FAD) 的合成,损害了能量生产.

结论:

通过损害精子尾巴内的能量供应,特别是通过抑制线粒体ATP合成,DEHP降低了精子的运动性.
该机制涉及干扰弗拉腺因二核酸 (FAD) 辅因子合成,并随后减少线粒体呼吸链活动.
这项研究强调精子尾作为DEHP诱导的生殖毒性的一个关键目标.

关键词:

(2-乙烯基) 甲酸盐 (DEHP) 是一种这就是FAD FAD.精子的运动性精子的运动性利博弗拉维因是一种黄素.精子的尾巴是精子的尾巴.

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