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BarH1调节了Dastarcus helophoroides中保存的气味结合蛋白22的表达

Rui-Nan Yang¹, Dong-Zhen Li², Ao Liu³

¹Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China; College of Bioscience and Resource Environment/Key Laboratory of Urban Agriculture (North China), Ministry of Agriculture and Rural Affairs of the People's Republic of China, Beijing University of Agriculture, Beijing, 102206, China.

Insect biochemistry and molecular biology

|January 29, 2025

概括

害虫和它们的自然敌人中的进化保存的气味结合蛋白 (OBPs) 具有相似的转录调节. 转录因子BarH1控制DhelOBP22的表达,影响昆虫的行为.

科学领域:

化学生态化学生态学
昆虫的行为昆虫的行为
分子生物学分子生物学

背景情况:

化学信号介导植物,食草动物和自然敌人之间的三性相互作用.
害虫 (例如,Monochamus alternatus) 和自然敌人 (例如,Dastarcus helophoroides) 中保存的气味结合蛋白 (OBPs) 识别宿主植物挥发物,对于害虫与敌人相互作用至关重要.

研究的目的:

调查害虫和它们的自然敌人的进化保存的OBPs是否具有类似的转录调节机制.
确定控制DhelOBP22在D. helophoroides中的表达的调控元素和因素.

主要方法:

染色体行走以确定DhelOBP22.22的促进子区域.
双露西法酶试验和电泳运动转移试验 (EMSA) 来确认转录因子结合.
通过RNA干扰 (RNAi) 来静止BarH1基因并评估其对DhelOBP22表达和昆虫行为的影响.

主要成果:

转录因子BarH1被确定为DhelOBP22表达的调节者,通过与其促进子区域结合.
通过RNAi抑制BarH1显著抑制了DhelOBP22的表达.
抑制DhelOBP22表达导致交配的雌性D. helophoroides对坎的吸引力丧失.

结论:

保存的转录调节机制参与控制进化保存的OBPs在草食动物和它们的自然敌人的表达.
这种保存的调节突出了一个潜在的共同进化策略,即调解三性相互作用.

关键词:

保存的转录因子条条.达斯塔克斯 (Dastarcus helophoroides) 是一种白的植物.进化过程中保存的气味结合蛋白质.转录调节的机制这种动物是Monochamus alternatus.

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