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通过直接激活玉米中的ZmPIN1基因,LG1促进了前带的形成

Zhuojun Zhong¹, Minhao Yao¹, Yingying Cao¹

¹State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangdong Laboratory for Lingnan Modern Agriculture, College of Life Sciences, South China Agricultural University, Guangzhou, Guangdong 510642, China.

Journal of genetics and genomics = Yi chuan xue bao

|January 29, 2025

概括

转录因子LG1通过直接激活auxin转运基因来调节玉米叶角. 这一发现为培育耐受高密度种植的玉米品种提供了目标.

科学领域:

植物生物学植物生物学
遗传学是一个遗传学.
农业科学农业科学

背景情况:

高植物密度增加了作物产量,需要建筑特征,如较小的叶角 (LA) 适应.
已知转录因子LG1的甲状腺结合蛋白 (SBP) 影响LA,但其调节机制尚不清楚.

研究的目的:

阐明LG1调节玉米叶角形成的分子机制.
确定参与LG1介导LA调节的关键基因和途径.

主要方法:

野生类型和lg1突变性玉米叶前带 (PLB) 区域的比较RNA测序.
基因本体学 (GO) 丰富分析以确定丰富的途径和转录因子.
分子实验包括促进体结合试验和突变体分析 (双重和三重突变体,过度表达).

主要成果:

在RNA-seq中确定了在植物激素通路中丰富的辅酶载体基因 (ZmPIN1a,ZmPIN1b,ZmPIN1c).
LG1直接与ZmPIN1a,ZmPIN1b和ZmPIN1c的转录结合并激活它们.
ZmPIN基因的突变减少了耳环大小和LA,而ZmPIN1a过度表达增加了耳环大小和LA.

结论:

通过直接激活辅酶载体基因,LG1调节PLB形成和玉米叶角.
这项研究确定了LG1,辅酶运输和LA之间的功能联系.
确定ZmPIN基因作为高密度耐受性玉米育种中LA遗传改进的有价值标.

关键词:

有助于运输的运输.一个LG1一个LG1 叶子的角度 (LA)玉米 (Zea mays L.) 是一种大豆.乐队的乐队是前乐队.

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