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在功能化的蒙莫里隆石上对双酶的辅助因子指导的联合不移,具有增强的催化性能

Ke Wang¹, Shiyong Sun¹, Sen Lin²

¹State Key Laboratory of Environment-Friendly Energy Materials, School of Materials and Chemistry, Southwest University of Science and Technology, Mianyang, Sichuan 621010, China; School of Environment and Resource, Key Laboratory of Solid Waste Treatment and Resource Recycle of Ministry of Education, Southwest University of Science and Technology, Mianyang, Sichuan 621010, China.

International journal of biological macromolecules

|January 29, 2025

概括

一种新型的双酶级联系统使用辅助因子指导的葡萄糖氧化酶 (GOx) 和胡卜氧化酶 (HRP) 联合固定到功能蒙莫里隆石 (FMt) 上,证明了可持续的酶催化剂的增强稳定性和催化效率.

科学领域:

生物催化剂是一种生物催化剂.
酶固定化酶固定化
材料科学材料科学材料科学

背景情况:

多酶级联催化提供了诸如高效率和减少副作用等优势.
酶固定对于提高酶的稳定性和可重复使用性至关重要.
开发用于定向酶联合固定化的有效方法仍然是一个挑战.

研究的目的:

通过辅助因子导向的定向性联合固定,开发一种新的双酶级联系统 (GOx-FMt-HRP).
提高固定酶的稳定性,可重复使用性和催化效率.
提供一种简单有效的双酶联合固定方法.

主要方法:

葡萄糖氧化酶 (GOx) 和胡卜素过氧化酶 (HRP) 的辅助因子导向的定向联合不运动到功能性蒙特莫里隆石 (FMt).
复制辅因子和阿波酶以实现特定的酶定向.
对催化稳定性,可重复使用性和对物理吸附和自由酶混合物的效率进行比较分析.

主要成果:

GOx-FMt-HRP系统显著增强了催化稳定性,在一个月后保持了79.3%的活性,而物理吸附率为60.4%,自由酶为1.32%.
固定酶在10次使用后保持了超过74%的活性,超过了物理吸附 (61%).
在GOx-FMt-HRP中基质通道导致葡萄糖 (2.80 S−1·mM−1) 和TMB (3.87 S−1·mM−1) 的最高催化效率,分别大约是自由酶的2.6倍和2.1倍.

结论:

辅助因子指导的定向性联合固定是提高酶性能的有效策略.
开发的GOx-FMt-HRP系统提供了卓越的稳定性,可重复使用性和催化效率.
这种方法提供了一种简单的双酶联合固定方法,促进了可持续的酶催化.

关键词:

双酶级联反应是一种双酶级联反应.酶定向的联合固定化.这是一种蒙莫里隆石 (Montmorillonite).

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