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准备,表征和稳定性基于乳清蛋白隔离的纳米颗粒与线粒体准能力

Chaoting Wen¹, Jialuo Tang¹, Maowei Wu¹

¹College of Food Science and Engineering, Yangzhou University, Yang Zhou 225127, China.

International journal of biological macromolecules

|January 29, 2025

概括

乳清蛋白分离物和点纳米颗粒有效封装奎尔,通过向癌细胞线粒体来增强其生物可用性和抗癌性质. 这改善了用于功能性食品的氨酸输送.

科学领域:

生物材料科学生物材料科学
纳米技术纳米技术
营养保健品营养保健品

背景情况:

奎尔 (Que) 是一种具有强烈抗癌活性的黄类化合物.
较差的水溶性和低的生物可用性极限. 在功能性食品中的应用.
开发有效的交付系统对于Que.是至关重要的. 使用率.利用率.使用率.

研究的目的:

使用乳清蛋白分离物 (WPI),trifenylphosphonium bromide (TPP) 和pectin (P) 开发和表征素载纳米粒子.
研究开发的纳米颗粒的形成机制,奎尔素释放动力学和体外抗瘤活性.
为了评估丁修饰的潜力,线粒体向的纳米载体增强Que. 交付,交付,交付,交付,交付,交付,交付,交付,交付,交付.

主要方法:

使用WPI,TPP,Que和P以最佳比例 (50:8:1:20 w/w/w/w) 制备纳米粒子.
纳米粒子大小,泽塔潜力和封装效率 (82.64%) 的表征.
在体外消化,释放动力学 (非Fickian扩散) 和HepG-2细胞中的细胞吸收研究.

主要成果:

优化WPI-TPP-Que-P纳米粒子的尺寸为261.7nm,泽塔电位为-42.1mV.
pectin的修饰增加了Que. 在体外消化后,保留率提高了4.03%.
纳米颗粒向HepG-2细胞线粒体,通过改变线粒体膜潜力和细胞因子水平 (IL-10,TNF-α) 来显著促进亡 (83.6%).

结论:

经过丁修饰的,针对线粒体的纳米载体显示出增强的奎尔素输送和体外抗癌疗效.
这一系统有望改善氨酸在功能性食品行业的应用.
这项研究突出了定制纳米载体在生物活性化合物输送方面的潜力.

关键词:

它具有抗瘤活性.在HepG-2细胞中.奎尔丁 (Quercetin) 是一种青素.释放动力学释放动力学化三烯 (Triphenylphosphonium bromide) 是一种化三烯 (Triphenylphosphonium bromide) 的一种化合物.

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