在准确和快速识别手术内副甲状腺激素的APTAMER-抗体双识别三明治SERRS生物传感器
Shanshan Xu1, Shi Wang2, Jun-Jie Wu1
1State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, College of Energy, Discipline of Intelligent Instrument and Equipment, Cancer Center and Department of Breast and Thyroid Surgery, Department of Ultrasound, Xiang'an Hospital of Xiamen University, School of Medicine, Laboratory Animal Center Xiamen University, Xiamen University, Xiamen 361005, China.
Analytical chemistry
|January 29, 2025
概括
这项研究引入了一种新的表面增强拉曼光谱法 (SERRS) 方法,用于快速内科检测甲状腺激素 (PTH). 这种技术通过识别副甲状腺来提高手术安全性,防止甲状腺功能低下和甲状腺癌手术后的低血症.
科学领域:
- 生物医学工程 生物医学工程
- 分析化学 分析化学
- 手术创新 在外科创新.
背景情况:
- 甲状腺癌手术有甲状腺侧甲状腺损伤的风险,导致甲状腺功能低下和低血症.
- 目前的方法缺乏立即的手术内反来识别副甲状腺.
- 改进的外科安全需要实时诊断工具来保持副甲状腺功能.
研究的目的:
- 开发一个快速,灵敏的平台,用于在手术期间检测甲状腺上腺激素 (PTH).
- 在甲状腺癌手术中提高手术精度和患者的治疗结果.
- 为在手术期间识别副甲状腺提供立即诊断工具.
主要方法:
- 使用表面增强拉曼光谱法 (SERRS) 与IR808染料进行增强的PTH检测.
- 为了特定的PTH识别,采用了阿普坦-抗体三明治试验.
- 优化了测试参数,包括化温度和试剂度.
主要成果:
- 实现了PTH检测的高灵敏度 (9.75 pg/mL),化时间为5分钟.
- 在临床样本上证明了SERRS测定性能,对CMIA显示94%的灵敏度和100%的特异性.
- 验证了该方法从体外研究到临床应用,超过横向流动生物传感器的性能.
结论:
- 开发的SERRS平台为手术内PTH监测提供了一种新,快速和准确的方法.
- 这项技术可以显著提高接受甲状腺癌手术的患者的安全性和生活质量.
- 这项研究为内分泌外科手术中的手术内诊断和治疗提供了一个新的范式.
相关概念视频
01:25Rapid Identification of Pathogens
MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...
01:24Automated Microbial Diagnostics
Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...