Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.0K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.0K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

6.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
6.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Untrained Position-Encoded Multilayer Perceptron Network for Structured Illumination Microscopy Reconstruction.

Chemical & biomedical imaging·2026
Same author

Optical super-resolution histology of formalin-fixed paraffin-embedded tissue samples: challenges and opportunities.

Nature communications·2025
Same author

Chip-based label-free incoherent super-resolution optical microscopy.

Light, science & applications·2025
Same author

Integrated Ultrasound Neuromodulation and Optical Neuroimaging in Awake Mice using a Transparent Ultrasound Transducer Cranial Window.

bioRxiv : the preprint server for biology·2025
Same author

Engineering plasmonic charge kinetics and broadband photoelectrochemical spectral responses using a multi-resonant Au-TiO<sub>2</sub> plasmonic particle grating-based optical resonator.

Nanoscale·2024
Same author

Plasmonic nano-bowls for monitoring intra-membrane changes in liposomes, and DNA-based nanocarriers in suspension.

Biomedical optics express·2024

相关实验视频

Updated: May 30, 2025

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

25.1K

同线性六合镜镜基多周期结构化照明显微镜.

Anupriya Tiwari1, Krishnendu Samanta1,2,3, Shital Devinder4

  • 1Department of Physics, Indian Institute of Technology Delhi, New Delhi 110016, India.

Nano letters
|January 29, 2025
PubMed
概括
此摘要是机器生成的。

这项研究介绍了多周期SIM (mMP-SIM),一种新的超高分辨率显微镜技术. 它在广的视野中实现了显著增强的分辨率,克服了以前光学纳米镜的局限性.

关键词:
光成像成像技术的使用.高通量成像技术的成像大大的视野.多周期照明多周期照明结构化照明显微镜结构化照明显微镜超级分辨率的超级分辨率基于传输的显微镜.

更多相关视频

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
08:53

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

9.7K
Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

9.8K

相关实验视频

Last Updated: May 30, 2025

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

25.1K
Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
08:53

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

9.7K
Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

9.8K

科学领域:

  • 光学显微镜的使用方法
  • 超高分辨率的纳米显微镜
  • 生物医学成像技术 生物医学成像技术

背景情况:

  • 结构化照明显微镜 (SIM) 提供了强大的广场光学纳米镜.
  • 当前的SIM方法在大视野 (FOV) 中实现高分辨率方面面临挑战.
  • 在保持大FOV的同时提高分辨率对于先进的生物成像至关重要.

研究的目的:

  • 开发一种新的SIM技术,在大型FOV上实现高分辨率.
  • 为了克服超分辨率显微镜中的空间带宽产品限制.
  • 为了展示一个可扩展的超高分辨率成像解决方案,用于生物样品.

主要方法:

  • 基于倾斜镜的多周期SIM (mMP-SIM) 的实施.
  • 通过六束干扰与定制的镜子安装生成多周期结构化图案.
  • 为可扩展的超高分辨率成像进行照明和收集路径的分离.

主要成果:

  • 通过20×/0.40 NA的客观镜头在一个大FOV (0.53mm × 0.34mm) 上实现了3.16倍的分辨率改进.
  • 显示了9.98倍的改进,克服了高空间带宽产品的挑战.
  • 通过使用28×/0.80 NA的客观镜头,在0.40mm × 0.25mm的面积上达到170nm的光学分辨率.

结论:

  • mMP-SIM有效地提高了大FOV超分辨率显微镜的分辨率.
  • 该技术克服了空间带宽的限制,使可扩展的超高分辨率成像成为可能.
  • 在光珠和U2OS细胞上的实验验证证证了该方法的实用性.