开发基于ceRNA的lncRNA-miRNA-mRNA调节网络,以揭示骨肌肉发育中的作用
Wang Wenlun1,2, Yu Chaohang1, Huang Yan1
1Department of Food Science and Engineering, Moutai Institute, Renhuai, Guizhou, China.
Frontiers in bioinformatics
|January 30, 2025
概括
长非编码RNAs (lncRNAs) 显著影响骨肌肉发育和缩. 这项研究确定了关键的lncRNA和参与肌肉生长,再生和微重力诱导的衰减的调节网络.
科学领域:
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
- 生物信息学是一种生物信息学.
背景情况:
- 长非编码RNAs (lncRNAs) 在骨肌肉发育和缩中的确切作用尚未完全理解.
- lncRNAs越来越多地被认为是它们在各种生物过程中的调节功能.
研究的目的:
- 通过生物信息分析阐明 lncRNAs 在骨肌肉发育,再生和缩中的作用.
- 构建和可视化 lncRNA-miRNA-mRNA 调节网络.
主要方法:
- 来自小鼠研究的26个GEO数据集的生物信息分析.
- 通过使用miRcode,IncBaseV2,miRDB和Targetscan.预测lncRNA-miRNA和miRNA-mRNA相互作用.
- 使用ggalluvial.com构建和可视化监管网络.
- 功能性丰富分析 (GO,Reactome,KEGG,GSEA). 功能性丰富分析 (GO,Reactome,KEGG,GSEA). 功能性丰富分析 (GO,Reactome,KEGG,GSEA). 功能性丰富分析 (GO,Reactome,KEGG,GSEA). 功能性丰富分析 (GO,Reactome,KEGG,GSEA). 功能性丰富分析 (GO,Reactome,KEGG,GSEA). 功能性丰富分析 (GO,Reactome,KEGG,GSEA).
主要成果:
- 鉴定了五种候选lncRNAs (Xist,Gas5,Pvt1,Airn,Meg3) 可能调解肌肉发育,再生和微重力诱导的衰减.
- 构建一个详细的lncRNA-miRNA-mRNA调节网络,包括特定的相互作用.
- 在肌肉过程中观察显著的信号通路变化 (例如PI3K/Akt,MAPK,NF-κB).
结论:
- lncRNAs在骨肌肉蛋白质合成,降解,增殖,分化,功能和新陈代谢中起着至关重要的作用.
- 这项研究为正常和微重力条件下的骨肌肉调节的分子机制提供了新的见解.
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