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对肠道酸化的进化见解:基底脊椎动物鱼中的无脊椎动物类机制

Alyssa M Weinrauch^1,2, Garfield T Kwan³, Marina Giacomin^1,2

¹Department of Biological Sciences, University of Alberta, 116 St and 85 Ave, Edmonton, AB, Canada, T6G 2R3.

The Journal of experimental biology

|January 30, 2025

概括

鱼,最基本的脊椎动物,使用囊泡型H+-ATPase (VHA) 进行肠酸化,与其他使用H+/K+-ATPase (HKA) 的脊椎动物不同. 这表明鱼具有独特的消化适应,保留无脊椎动物类的VHA功能,同时表现出一些胰腺和肠道的作用.

科学领域:

比较生理学比较生理学
进化生物学是进化的生物学.
胃肠病学胃肠病学

背景情况:

消化系统的酸性化对于甲基动物的营养吸收和免疫功能至关重要.
脊椎动物利用胃H+/K+-ATPase (HKA) 进行肠道酸化,这种适应与胃的进化有关.
鱼的消化酸性化机制,最基本的脊椎动物,仍然在很大程度上未知.

研究的目的:

为了研究太平洋鱼 (Eptatretus stoutii) 的消化系统酸化机制.
确定鱼中H+/K+-ATPase (HKA) 的存在或不存在,从而阐明其进化史.
探索囊泡型H+-ATPase (VHA) 在鱼肠道生理学中的作用及其与胰腺和肠道功能潜在的重叠.

主要方法:

对E. stoutii肠道转录组和E. burgerii基因组进行分析,以确定HKA基因.
在鱼肠道组织中定位VHA和可溶性腺酸环酶的免疫覆盖.
使用孤立的鱼肠袋测量H+分泌和其通过cAMP通路的刺激的功能性测试.

主要成果:

鱼利用VHA进行肠道酸化,缺乏HKA,支持其在2R全基因组复制后出现.
VHA在肠细胞顶膜和下顶膜区域显著表达,与可溶性腺环酶一起.
鱼肠囊表现出由cAMP刺激的VHA-依赖的H+分泌量增加,VHA存在于不成熟的,但不成熟的,胰腺样生殖原粒中.

结论:

鱼拥有一种依赖于VHA的肠酸化系统,类似于无脊椎动物.
鱼表现出胰腺和肠道的功能特征,尽管缺乏真正的胃和HKA.
这些发现突出了鱼独特的消化适应,桥梁无脊椎动物和脊椎动物的消化策略.

关键词:

亚丁烯基基环酸酶是什么?阿格纳坦·阿格纳坦巴菲洛米辛 (Bafilomycine) 是一种吃饭后的时间吃饭后的时间质子是一个质子.这就是Zymogen.

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