相关实验视频
Updated: May 30, 2025

10:12
Visualization of G3BP Stress Granules Dynamics in Live Primary Cells
Published on: May 21, 2014
18.3K
G3BP1核糖蛋白复合体调节焦点粘附蛋白的移动性和细胞迁移
Liana C Boraas1, Mengwei Hu2, Pieter Martino1
1Yale Cardiovascular Research Center, Department of Internal Medicine, Section of Cardiology, Yale University School of Medicine, New Haven, CT 06511, USA; Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA.
Cell reports
|January 30, 2025
概括
这项研究表明,压力颗粒RNA结合蛋白 (RBPs) 和它们相关的mRNA定位到焦点粘附,通过调节焦点粘附大小和蛋白质移动性来增强细胞迁移.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 亚细胞mRNA定位对于细胞迁移等生物过程至关重要.
- 已知β-actin mRNA和ZBP1/IGF2BP1可定位到焦点粘附点 (FA),以支持细胞迁移.
- 在FA中存在其他mRNA和RBP的存在在很大程度上仍未被探索.
研究的目的:
- 识别在焦点粘附点 (FA) 定位的mRNA和RNA结合蛋白 (RBPs).
- 阐明这些分子调节FA功能和细胞迁移的机制.
- 在非压力条件下研究含有G3BP1的核糖蛋白复合体 (RNP) 的作用.
主要方法:
- 使用转录组分析识别了数百个在FA中丰富的mRNA (FA-mRNAs).
- 关于FA-mRNA与压力颗粒 (SG) mRNA和SG RBP相关性的表征.
- 研究G3BP1对FA蛋白的RNA依赖结合及其域在RNP形成和FA局部化中的作用.
主要成果:
- 数百个FA-mRNA被确定,与压力颗粒 (SG) mRNA共享特征.
- 发现SG RBP G3BP1以RNA依赖的方式与FA蛋白结合.
- G3BP1的RNA结合和二元化域对其FA局部化和细胞迁移至关重要.
- G3BP1 RNPs通过增加FA蛋白的移动性和FA大小来提高细胞速度.
结论:
- 含有G3BP1的RNP被招募到FA中,并在调节FA动态方面发挥重要作用.
- 这种由G3BP1 RNPs招募和随后调节的FA蛋白对于有效的细胞迁移至关重要.
- 这些发现突出了G3BP1 RNPs在正常生理条件下控制FA功能的新作用,超出了它们在压力颗粒中的已知的作用.
相关概念视频
Intracellular Signaling Affects Focal Adhesions
2.5K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Some...
2.5K
Cytoskeletal Coordination in Cell Migration
4.7K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
4.7K
GTPases and their Regulation
8.2K
Guanine nucleotide-binding proteins (G-proteins), also known as GTPases, are a superfamily of proteins that regulate many cellular processes, such as cell signaling, vesicular transport, and the regulation of cell shape and motility. Mutation or dysfunction of these proteins can lead to disease. There are around 40,000 known G-proteins that can broadly be classified into two groups ‒ small G-proteins consisting of a single domain and large multi-domain G-proteins.
Large G-proteins,...
Large G-proteins,...
8.2K
Small GTPases - Ras and Rho
3.9K
Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
Three regulatory proteins control their activity:
3.9K
Coat Assembly and GTPases
3.5K
Vesicles incorporate different coat protein subunits in different cell locations, which changes the properties of the coat, such as the shape and geometry of the transport vesicles. Thus, vesicle coat proteins also play a significant role in cargo selection.
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
3.5K
Mechanism of Filopodia Formation
2.3K
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
2.3K

