基于基因组的Yersinia enterocolitica从2024年瑞士的不同食物矩阵的基因组表征
Marc J A Stevens1, Karen Barmettler1, Lucien Kelbert1
1Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
概括
在瑞士的猪肉,肉和农产品中发现了Yersinia enterocolitica菌株. 全基因组测序揭示了遗传多样性,其中一些菌株携带毒性因子,但没有抗菌素耐药性基因,并显示与人类分离物有联系.
科学领域:
- 食品微生物学 食品微生物学
- 基因组学就是基因组学.
- 病原体监测 病原体监测 病原体监测
背景情况:
- 耶尔西尼亚肠结肠杆菌是食源性胃肠炎的一个重要原因.
- 关于Y. enterocolitica在各种食品中的遗传多样性和病原性潜力的知识有限.
- 了解这些方面对于公共卫生和食品安全至关重要.
研究的目的:
- 在瑞士的零售食品样本中调查Yersinia enterocolitica的发生,遗传多样性和病原性潜力.
- 用全基因组测序 (WGS) 来表征Y. enterocolitica分离物.
- 为了将食源性分离物与人类Y. enterocolitica菌株进行比较.
主要方法:
- 据推测,Y. enterocolitica菌株从瑞士零售商采集的猪肉,肉和农产品样本中分离出来.
- 全基因组测序 (WGS) 在所有分离物中进行.
- 基因组分析包括生物类型,序列类型和等离子体分析.
主要成果:
- 在猪肉 (32%),肉 (25%) 和农产品 (22%) 中检测到Yersinia enterocolitica.
- 确定了45种序列类型 (ST),其中大多数分离物属于生物型 (BT) 1A,3个属于BT 4 (来自猪肉).
- BT 4分离物携带具有毒性因子的pYV类等离子体;一些分离物携带具有氧化应激耐受性,细胞毒性或耐热性肠毒素基因的等离子体. 没有检测到等离子体介导的抗微生物耐药性基因.
- Y. enterocolitica BT 4和BT 1A分离物在遗传上与之前确定的瑞士人类菌株相似.
结论:
- 这项研究为瑞士食品商品中的Y. enterocolitica提供了全面的基因组洞察力.
- 这些发现突出了在食品中存在各种Y. enterocolitica菌株的存在,它们在食物中具有不同的致病潜力.
- 食品和人类分离物之间的遗传关系强调了食品安全措施在预防Yersinia enterocolitica感染方面的重要性.
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