鱼肠共生细菌 Bacillus thuringiensis:RSM优化其细胞外脂酶生产,脂酶蛋白净化,表征和对接分析
Arul Dhayalan1, Kamala Jayanthi P D1, Sumathi Manoharan1
1ICAR-Indian Institute of Horticulture Research, Department of Entomology & Nematology, Hessaraghatta Lake Post, Bengaluru 560089, Karnataka, India.
International journal of biological macromolecules
|January 30, 2025
概括
从鱼肠中分离出产生脂酶的细菌,其中Bacillus thuringiensis (SS5) 显示出高活性. 优化的条件增强了脂酶的产生,揭示了生物柴油等工业应用的潜力.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 酶学 是一种酶学.
背景情况:
- 脂酶酶对于消化和新陈代谢至关重要.
- 主体肠道中的共生细菌是丰富的酶和营养素来源.
- 鱼的肠道微生物群代表了微生物酶的未被探索的储存库.
研究的目的:
- 从Systomus sarana gut中分离和识别产生脂酶的细菌.
- 使用响应表面方法 (RSM) 来优化脂酶的产生.
- 描述脂酶的特征,并评估其工业潜力.
主要方法:
- 通过16S rRNA测序进行细菌分离和鉴定.
- 使用RSM进行脂酶活性选和优化.
- 使用SDS-PAGE,FTIR,MALDI-TOF/MS和同质模型进行蛋白质表征.
- 用脂肪酸和tripalmitin进行分子对接研究.
主要成果:
- 隔离了11种细菌菌株;Bacillus thuringiensis (SS5) 显示了最高的脂酶指数 (5.46毫米) 和活性 (109 U/mL).
- 通过RSM优化 (pH 7.5,35°C,30小时,2.3%的,2.34%的乳糖) 产生了210 U/mL的脂酶.
- 鉴定揭示了一种~30kDa的蛋白质,具有特定的功能组和序列;分子对接显示出强烈的结合亲缘关系.
结论:
- 来自鱼肠的Bacillus thuringiensis是一种有前途的脂酶来源.
- 优化生产可以提高生物技术应用的酶产量.
- 描述的脂酶具有生物修复和生物柴油生产的潜力.
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