SLC29A1和SLC29A2是人类的尼古丁胺胺细胞膜载体
Mingyang Chen1,2, Luexiang Yuan1, Binxin Chen1,3
1College of Pharmaceutical Sciences, National Key Laboratory of Advanced Drug Delivery and Release Systems, Zhejiang University, Hangzhou, China.
Nature communications
|January 30, 2025
概括
平衡性核酸转运体1和2 (ENT1/2) 促进细胞尼古丁胺胺 (NAM) 的吸收,维持NAD+的稳态. 这些载体通过控制NAM水平和影响NAD+池来调节细胞呼吸和衰老.
科学领域:
- 细胞生物学 细胞生物学
- 代谢生物化学 代谢生物化学
背景情况:
- 尼古丁胺 (NAM) 是细胞能量和呼吸的关键NAD+前体.
- 虽然存在其他NAD+前体的载体,但细胞NAM吸收的机制仍然未知.
研究的目的:
- 为了确定负责尼古丁胺胺吸收的细胞载体.
- 阐明这种载体在调节细胞代谢,呼吸和衰老中的作用.
主要方法:
- 研究了平衡性核酸载体1和2 (ENT1和ENT2) 在细胞尼古丁胺胺吸收中的作用.
- 在ENT1/2操纵后,分析了细胞代谢物组成和基因表达的变化.
- 评估了ENTT1/2对细胞呼吸,线粒体状态和衰老的影响.
- 使用尼古丁胺胺 mononucleotide (NMN) 补充剂来逆转观察到的影响.
主要成果:
- ENT1和ENT2被确定为驱动细胞尼古丁胺胺吸收的主要载体.
- ENT1/2活动显著改变了细胞代谢物概况和基因表达,特别是尼古丁胺相关的转录.
- 通过影响NAD+水平和线粒体功能,对ENT1/2的抑制损害了细胞呼吸,并促进了衰老.
- 补充NMN可以逆转ENT1/2对呼吸,线粒体状态和衰老的影响.
结论:
- ENT1和ENT2是细胞尼古丁胺胺吸收和恒温的关键调节者.
- 这些载体在维持NAD+水平,细胞呼吸和预防衰老方面发挥着关键作用.
- 通过它们的传输活动,ENT1/2功能既是尼古丁胺水平保持者,也是生物调节者.
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