细胞粘附和通过微管体依赖的机械传导在流体膜上扩散
Oleg Mikhajlov1,2,3, Ram M Adar4,5,6, Maria Tătulea-Codrean4,5,7
1Institut Curie, Université PSL, Sorbonne Université, CNRS UMR168, Laboratoire Physico-Chimie Curie, 75005, Paris, France. oleg.mikhajlov@unige.ch.
Nature communications
|January 30, 2025
概括
细胞可以通过使用微管体力量在液体表面传播,而不仅仅是actomyosin收缩. 这揭示了微管在细胞粘附和在流体基板上的整合素聚类中的新机械作用.
科学领域:
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
- 机械生物学 机械生物学
背景情况:
- 集成蛋白集群对于细胞粘附,机械转导和生化信号传递至关重要.
- 研究通常集中在刚性基板上,忽视了像支持脂质双层 (SLBs) 这样的流体表面的行为.
- 在流体SLB上,移动整合素连接体挑战了细胞扩散和粘合复合体固的传统模型.
研究的目的:
- 研究在流体SLBs上的细胞扩散和整合蛋白聚类.
- 为了确定控制在流体基板上的细胞粘附和机械传导的力量.
- 阐明微管在流体环境上的整合蛋白聚类中的作用.
主要方法:
- 使用支持的脂质双层 (SLBs) 与Invasin,一种高亲和度整合素连接体功能化.
- 在Invasin-SLB上比较整体蛋白聚类和细胞扩散,与RGD功能化的SLB和玻璃基板进行比较.
- 采用理论模型来分析细胞粘附所涉及的力量.
主要成果:
- 细胞成功地在Invasin-SLB上扩散,形成复杂的整合蛋白,与玻璃上的相似.
- 与主导着actomyosin收缩的刚性基质不同,细胞在流体SLB上扩散取决于dynein介导的微管拉力.
- 微管也被发现可以推动粘合复合物,有助于粘合成熟.
结论:
- 在流体SLB上,细胞扩散和整合素机械传导由微管基于的力量调节,包括dynein拉动和微管推进.
- 这些发现凸显了以前低估的微管在整合素聚类中的机械作用.
- 流体基板系统可以揭示与流体和不可变形表面相关的力机制.
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