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复杂生物流体的生物标志物分析由芯片上的化学修饰光控制垂直纳米柱阵列装置进行.

Lanka Tata Rao^1,2, Adva Raz³, Fernando Patolsky^4,5

¹Center for Nanoscience and Nanotechnology, Tel Aviv University, Tel Aviv-Yafo, Israel.

Nature protocols

|January 30, 2025

概括

研究人员开发了纳米柱 (SiNP) 阵列,用于快速,可重复使用和定量捕获和释放生物标志物. 这项技术可以在芯片上对各种生物和化学物种进行多重分析,用于诊断和药物发现.

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科学领域:

* 纳米技术的使用
* 表面的化学成分
*生物分子工程 *生物分子工程

背景情况:

*纳米结构设备在疾病诊断和药物发现方面提供了多方面的应用.
*有效捕获和释放生物标志物对于敏感和定量分析至关重要.
* 现有的方法往往缺乏高级应用所需的速度,可重复使用性和多重复合功能.

研究的目的:

* 开发一种标准化的协议,用于生产用于生物标志物捕获和释放的纳米柱 (SiNP) 阵列.
* 为了实现生物分子的快速,可重复使用和定量在芯片上的多重分析.
* 展示SiNP阵列在分析多种生物和化学物种方面的实用性.

主要方法:

*制造单区域和多区域垂直纳米柱阵列.
* SiNP数组的形态特征.
* 用于生物感应器固定和光诱导释放捕获剂的化学修饰.
*使用绿色光蛋白作为模型生物标记物的演示,使用微板阅读器.

主要成果:

*为SiNP阵列制造和功能化建立了一个标准化的协议.
* SiNP平台展示了生物标志物的快速,可重复使用和定量捕获和释放.
* 生物样本中的生物分子的多重捕获和释放取得了成功.
* 协议可以在45小时内完成.

结论:

* 垂直纳米柱阵列为芯片上多重生物标志物分析提供了多功能平台.
* 开发的协议促进了可重复使用和定量捕获释放设备的生产.
* 这项技术在诊断,药物查和分析各种生物和化学物种方面具有很大的应用潜力.